Bilayer lipid membranes from falling droplets
Bilayer lipid membranes from falling droplets
We describe a system that provides a rapid and simple way of forming suspended lipid bilayers within a microfluidic platform from an aqueous droplet. Bilayer lipid membranes are created in a polymeric device by contacting monolayers formed at a two-phase liquid-liquid interface. Microdroplets, containing membrane proteins, are injected onto an electrode positioned above an aperture machined through a conical cavity that is filled with a lipid-alkane solution. The formation of the BLM depends solely on the device geometry and leads to spontaneous formation of lipid bilayers simply by dispensing droplets of buffer. When an aqueous droplet containing transmembrane proteins or proteoliposomes is injected, straightforward electrophysiology measurements are possible. This method is suitable for incorporation into lab-on-a-chip devices and allows for buffer exchange and electrical measurements.
1601-1605
Zagnoni, Michele
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Sandison, Mairi E.
90477e5a-2af6-44b1-8ea8-ae4515594c60
Marius, Phedra
ea2b2803-ef34-4e41-a39a-be75900f8aaf
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
2009
Zagnoni, Michele
abfb96d5-0d3c-4b23-864b-b1212f6d5a42
Sandison, Mairi E.
90477e5a-2af6-44b1-8ea8-ae4515594c60
Marius, Phedra
ea2b2803-ef34-4e41-a39a-be75900f8aaf
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Zagnoni, Michele, Sandison, Mairi E., Marius, Phedra and Morgan, Hywel
(2009)
Bilayer lipid membranes from falling droplets.
Analytical and Bioanalytical Chemistry, 393, .
Abstract
We describe a system that provides a rapid and simple way of forming suspended lipid bilayers within a microfluidic platform from an aqueous droplet. Bilayer lipid membranes are created in a polymeric device by contacting monolayers formed at a two-phase liquid-liquid interface. Microdroplets, containing membrane proteins, are injected onto an electrode positioned above an aperture machined through a conical cavity that is filled with a lipid-alkane solution. The formation of the BLM depends solely on the device geometry and leads to spontaneous formation of lipid bilayers simply by dispensing droplets of buffer. When an aqueous droplet containing transmembrane proteins or proteoliposomes is injected, straightforward electrophysiology measurements are possible. This method is suitable for incorporation into lab-on-a-chip devices and allows for buffer exchange and electrical measurements.
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Published date: 2009
Additional Information:
Imported from ISI Web of Science
Organisations:
Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 269924
URI: http://eprints.soton.ac.uk/id/eprint/269924
ISSN: 1618-2642
PURE UUID: 53f796b3-ccca-4da8-b148-26d0426eac68
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Date deposited: 21 Apr 2010 07:46
Last modified: 08 Jan 2022 02:59
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Contributors
Author:
Michele Zagnoni
Author:
Mairi E. Sandison
Author:
Phedra Marius
Author:
Hywel Morgan
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