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Effects of blood flow on the in vivo recovery of a small diffusible molecule by microdialysis in human skin

Effects of blood flow on the in vivo recovery of a small diffusible molecule by microdialysis in human skin
Effects of blood flow on the in vivo recovery of a small diffusible molecule by microdialysis in human skin
The aim of this study was to investigate the impact of changes in local blood flow on the recovery of a small, diffusible molecule (sodium fluorescein) from the extravascular tissue space of the skin, by microdialysis in vivo. Loss and recovery of fluorescein by linear microdialysis probes (5-kDa molecular mass cutoff, 0.2 mm diameter) inserted 1 mm apart in pairs, at three sites in the skin of the volar surface of the forearm of healthy volunteers, was measured under conditions of basal, reduced (noradrenaline, 0.005 mg/ml), and increased (glyceryl trinitrate, patch) blood flow.
Whereas loss of tracer from the delivery probe appeared unaffected by changes in local blood flow, retrieval of fluorescein by the second probe was directly related to blood flux, measured using scanning laser Doppler imaging. Steady-state recovery at vasoconstricted sites was 4.0 ± 0.7 µg · ml1 compared with 1.8 ± 0.7 µg · ml1 at control sites (p < 0.001). Local vasodilatation reduced the retrieval of fluorescein by ~50% to give a steady-state concentration of fluorescein in the dialysate at 40 to 50 min after the start of perfusion of 0.9 ± 0.3 µg · ml1 (p = 0.05). These studies in the skin are consistent with microdialysis theory. They suggest that clearance of solute by the blood will have a significant impact on microdialysis probe recovery and that, in the skin, the magnitude of this clearance is directly related to blood flow.
0022-3565
681-686
Clough, G.F.
9f19639e-a929-4976-ac35-259f9011c494
Boutsiouki, P.
d509b587-178d-4c09-beb4-5a524c88a797
Church, M.K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Michel, C.C.
2d98f230-d4a9-42b3-8f60-0f33f54a2556
Clough, G.F.
9f19639e-a929-4976-ac35-259f9011c494
Boutsiouki, P.
d509b587-178d-4c09-beb4-5a524c88a797
Church, M.K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Michel, C.C.
2d98f230-d4a9-42b3-8f60-0f33f54a2556

Clough, G.F., Boutsiouki, P., Church, M.K. and Michel, C.C. (2002) Effects of blood flow on the in vivo recovery of a small diffusible molecule by microdialysis in human skin. The Journal of Pharmacology and Experimental Therapeutics, 302 (2), 681-686.

Record type: Article

Abstract

The aim of this study was to investigate the impact of changes in local blood flow on the recovery of a small, diffusible molecule (sodium fluorescein) from the extravascular tissue space of the skin, by microdialysis in vivo. Loss and recovery of fluorescein by linear microdialysis probes (5-kDa molecular mass cutoff, 0.2 mm diameter) inserted 1 mm apart in pairs, at three sites in the skin of the volar surface of the forearm of healthy volunteers, was measured under conditions of basal, reduced (noradrenaline, 0.005 mg/ml), and increased (glyceryl trinitrate, patch) blood flow.
Whereas loss of tracer from the delivery probe appeared unaffected by changes in local blood flow, retrieval of fluorescein by the second probe was directly related to blood flux, measured using scanning laser Doppler imaging. Steady-state recovery at vasoconstricted sites was 4.0 ± 0.7 µg · ml1 compared with 1.8 ± 0.7 µg · ml1 at control sites (p < 0.001). Local vasodilatation reduced the retrieval of fluorescein by ~50% to give a steady-state concentration of fluorescein in the dialysate at 40 to 50 min after the start of perfusion of 0.9 ± 0.3 µg · ml1 (p = 0.05). These studies in the skin are consistent with microdialysis theory. They suggest that clearance of solute by the blood will have a significant impact on microdialysis probe recovery and that, in the skin, the magnitude of this clearance is directly related to blood flow.

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Published date: 2002

Identifiers

Local EPrints ID: 26995
URI: http://eprints.soton.ac.uk/id/eprint/26995
ISSN: 0022-3565
PURE UUID: 8ab61e18-1824-43e1-bba7-b993efbc50f9
ORCID for G.F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

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Date deposited: 25 Apr 2006
Last modified: 09 Jan 2022 02:51

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Contributors

Author: G.F. Clough ORCID iD
Author: P. Boutsiouki
Author: M.K. Church
Author: C.C. Michel

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