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Recombinant meningococcal PorA protein, expressed using a vector system with potential for human vaccination, induces a bactericidal immune response

Recombinant meningococcal PorA protein, expressed using a vector system with potential for human vaccination, induces a bactericidal immune response
Recombinant meningococcal PorA protein, expressed using a vector system with potential for human vaccination, induces a bactericidal immune response
The PorA protein of Neisseria meningitidis (subtype P1.7,16) was expressed as a recombinant protein using three vectors; pTWIN, pQE30 and pRSETA, which introduce different sized N-terminal leader sequences to the mature protein. The immunogenicity of these proteins was compared following incorporation into liposomes and ZW-micelles. All of the recombinant PorA (rPorA) preparations induced high titres of antibody that recognised the homologous PorA within the outer membrane (OM) and on the surface of meningococci. Antisera raised against liposomes and micelles containing the different rPorA proteins induced high and comparable levels of complement-mediated killing of the homologous, but not heterologous, strain. Furthermore, the bactericidal effect was greater when rPorA were incorporated into liposomes rather than detergent micelles. The minimal addition of three N-terminal amino acids in rPorA purified from the pTWIN vector represents a significant improvement over rPorA purified from vectors pQE30 and pRSETA, plus other previously purified rPorA, when considering use of these proteins in vaccines for human use.
Neisseria meningitidis, recombinant PorA, immunogenicity
1564-1569
Humphries, Holly E.
dfbfbf86-71b7-4a11-8192-0afd0eb817a6
Williams, Jeanette N.
b8cee39f-8709-40ac-84ad-e62e3c1c7d8e
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Heckels, John E.
fcfcfafe-5ca8-4728-9c5e-cb67f9af7e31
Humphries, Holly E.
dfbfbf86-71b7-4a11-8192-0afd0eb817a6
Williams, Jeanette N.
b8cee39f-8709-40ac-84ad-e62e3c1c7d8e
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Heckels, John E.
fcfcfafe-5ca8-4728-9c5e-cb67f9af7e31

Humphries, Holly E., Williams, Jeanette N., Christodoulides, Myron and Heckels, John E. (2004) Recombinant meningococcal PorA protein, expressed using a vector system with potential for human vaccination, induces a bactericidal immune response. Vaccine, 22 (11-12), 1564-1569. (doi:10.1016/j.vaccine.2003.09.042).

Record type: Article

Abstract

The PorA protein of Neisseria meningitidis (subtype P1.7,16) was expressed as a recombinant protein using three vectors; pTWIN, pQE30 and pRSETA, which introduce different sized N-terminal leader sequences to the mature protein. The immunogenicity of these proteins was compared following incorporation into liposomes and ZW-micelles. All of the recombinant PorA (rPorA) preparations induced high titres of antibody that recognised the homologous PorA within the outer membrane (OM) and on the surface of meningococci. Antisera raised against liposomes and micelles containing the different rPorA proteins induced high and comparable levels of complement-mediated killing of the homologous, but not heterologous, strain. Furthermore, the bactericidal effect was greater when rPorA were incorporated into liposomes rather than detergent micelles. The minimal addition of three N-terminal amino acids in rPorA purified from the pTWIN vector represents a significant improvement over rPorA purified from vectors pQE30 and pRSETA, plus other previously purified rPorA, when considering use of these proteins in vaccines for human use.

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More information

Published date: 2004
Keywords: Neisseria meningitidis, recombinant PorA, immunogenicity

Identifiers

Local EPrints ID: 27161
URI: http://eprints.soton.ac.uk/id/eprint/27161
PURE UUID: 986b3f23-6785-456a-a378-765a462b1b84
ORCID for Myron Christodoulides: ORCID iD orcid.org/0000-0002-9663-4731

Catalogue record

Date deposited: 26 Apr 2006
Last modified: 16 Mar 2024 02:38

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Contributors

Author: Holly E. Humphries
Author: Jeanette N. Williams
Author: John E. Heckels

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