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Altered phospholipid composition and aggregate structure of lung surfactant is associated with impaired lung function in young children with respiratory infections

Altered phospholipid composition and aggregate structure of lung surfactant is associated with impaired lung function in young children with respiratory infections
Altered phospholipid composition and aggregate structure of lung surfactant is associated with impaired lung function in young children with respiratory infections
Alterations to pulmonary surfactant structure, composition, and function contribute to the severity of respiratory infections. Analysis of bronchoalveolar lavage fluid (BALF) from children undergoing diagnostic bronchoscopy for structural abnormalities (control group, n = 24), asthma (n = 18), lung infection (n = 30), and cystic fibrosis (CF, n = 15) showed that BALF phospholipid concentration decreased with age for the control group and was elevated in all disease groups. The fractional concentration of the major surface active component, dipalmitoyl phosphatidylcholine (PC16:0/16:0), correlated (r2 = 0.608, P < 0.01) with airway resistance (FEV1% predicted), and decreased PC16:0/16:0 was accompanied by increased concentrations of phospholipid components characteristic of cell membranes (PC16:0/18:1 and PI18:0/20:4). Median minimal surface tension, measured by pulsating bubble surfactometer, was elevated (P < 0.01) in both infection (17.5 mN/m) and CF (17.1 mN/m) compared with the control group (1.5 mN/m). Centrifugation (60,000 x g, 40 min) of BALF indicated that infection was accompanied by accumulation of large aggregate forms of surfactant, in contrast to previous reports of increased conversion to inactive small aggregate surfactant particles in ventilated patients with respiratory failure. This accumulation of surface-inactive, large aggregate forms of surfactant, possibly due to mixing with membrane material from inflammatory cells, may contribute to severity of lung disease in children with respiratory infections.
1044-1549
714-721
Mander, Ann
2fcb3a33-6bb8-4463-8b06-79cd508e9d80
Langton-Hewer, Simon
07f6692b-f861-4eec-8e46-4e0acce1154f
Bernhard, Wolfgang
a93ed9d7-1f32-42e8-bf9c-c9f0f14ad2a2
Warner, John O.
50630e99-8486-4859-ade3-cd2c79c5a153
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Mander, Ann
2fcb3a33-6bb8-4463-8b06-79cd508e9d80
Langton-Hewer, Simon
07f6692b-f861-4eec-8e46-4e0acce1154f
Bernhard, Wolfgang
a93ed9d7-1f32-42e8-bf9c-c9f0f14ad2a2
Warner, John O.
50630e99-8486-4859-ade3-cd2c79c5a153
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66

Mander, Ann, Langton-Hewer, Simon, Bernhard, Wolfgang, Warner, John O. and Postle, Anthony D. (2002) Altered phospholipid composition and aggregate structure of lung surfactant is associated with impaired lung function in young children with respiratory infections. American Journal of Respiratory Cell and Molecular Biology, 27 (6), 714-721. (doi:10.1165/rcmb.4746).

Record type: Article

Abstract

Alterations to pulmonary surfactant structure, composition, and function contribute to the severity of respiratory infections. Analysis of bronchoalveolar lavage fluid (BALF) from children undergoing diagnostic bronchoscopy for structural abnormalities (control group, n = 24), asthma (n = 18), lung infection (n = 30), and cystic fibrosis (CF, n = 15) showed that BALF phospholipid concentration decreased with age for the control group and was elevated in all disease groups. The fractional concentration of the major surface active component, dipalmitoyl phosphatidylcholine (PC16:0/16:0), correlated (r2 = 0.608, P < 0.01) with airway resistance (FEV1% predicted), and decreased PC16:0/16:0 was accompanied by increased concentrations of phospholipid components characteristic of cell membranes (PC16:0/18:1 and PI18:0/20:4). Median minimal surface tension, measured by pulsating bubble surfactometer, was elevated (P < 0.01) in both infection (17.5 mN/m) and CF (17.1 mN/m) compared with the control group (1.5 mN/m). Centrifugation (60,000 x g, 40 min) of BALF indicated that infection was accompanied by accumulation of large aggregate forms of surfactant, in contrast to previous reports of increased conversion to inactive small aggregate surfactant particles in ventilated patients with respiratory failure. This accumulation of surface-inactive, large aggregate forms of surfactant, possibly due to mixing with membrane material from inflammatory cells, may contribute to severity of lung disease in children with respiratory infections.

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Published date: 2002

Identifiers

Local EPrints ID: 27249
URI: http://eprints.soton.ac.uk/id/eprint/27249
ISSN: 1044-1549
PURE UUID: c29a639c-e6e7-471b-aa6b-479dbcfc29b6
ORCID for Anthony D. Postle: ORCID iD orcid.org/0000-0001-7361-0756

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Date deposited: 28 Apr 2006
Last modified: 16 Mar 2024 02:32

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Contributors

Author: Ann Mander
Author: Simon Langton-Hewer
Author: Wolfgang Bernhard
Author: John O. Warner

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