Probing the interaction of lipids with the non-annular binding sites of KcsA by magic-angle spinning NMR
Marius, P., de Planque, M.R.R. and Williamson, P.T.F. (2012) Probing the interaction of lipids with the non-annular binding sites of KcsA by magic-angle spinning NMR. Biochimica et Biophysica Acta, 1818, (1), 90-96.
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Description/Abstract
The activity of the potassium channel, KcsA is tightly regulated through the interactions of anionic lipids with high-affinity non-annular lipid binding sites located at the interface between the channel’s subunits. Here we present solid-state phosphorous NMR studies that resolve the negatively charged lipid phosphatidylglycerol within the non-annular lipid-binding site. Perturbations in chemical shift observed upon the binding of phosphatidylglycerol are indicative of the interaction of positively charged sidechains within the non-annular binding site and the negatively charged lipid headgroup. Site directed mutagenesis studies have attributed these charge interactions to R64 and R89. Functionally the removal of the positive charges from R64 and R89 appears to act synergistically to reduce the probability of channel opening.
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Physical and Applied Science > Electronics and Computer Science > NANO |
| Item ID: | 272758 |
| Date Deposited: | 09 Sep 2011 09:45 |
| Last Modified: | 25 Aug 2012 23:35 |
| Contributors: | Marius, P. (Author) de Planque, M.R.R. (Author) Williamson, P.T.F. (Author) |
| Date: | January 2012 |
| Status: | Published |
| Further Information: | Google Scholar |
| ISI Citation Count: | 2 |
| URI: | http://eprints.soton.ac.uk/id/eprint/272758 |
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