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A defective type 1 response to rhinovirus in atopic asthma

A defective type 1 response to rhinovirus in atopic asthma
A defective type 1 response to rhinovirus in atopic asthma
Background: Rhinoviruses (RVs) are the most frequent precipitants of the common cold and asthma exacerbations, but little is known about the immune response to these viruses and its potential implications in the pathogenesis of asthma.
Methods: Peripheral blood mononuclear cells (PBMC) from patients with atopic asthma and normal subjects were exposed to live or inactivated RV preparations. Levels of interferon (IFN)? and interleukins IL-12, IL-10, IL-4, IL-5 and IL-13 were evaluated in the culture supernatants with specific immunoassays.
Results: Exposure of PBMC to RVs induced the production of IFN?, IL-12, IL-10, and IL-13. Cells from asthmatic subjects produced significantly lower levels of IFN? and IL-12 and higher levels of IL-10 than normal subjects. IL-4 was induced only in the asthmatic group, while the IFN?/IL-4 ratio was more than three times lower in the asthmatic group.
Conclusions: This evidence suggests that the immune response to RVs is not uniquely of a type 1 phenotype, as previously suggested. The type 1 response is defective in atopic asthmatic individuals, with a shift towards a type 2 phenotype in a way similar, but not identical, to their aberrant response to allergens. A defective type 1 immune response to RVs may be implicated in the pathogenesis of virus induced exacerbations of asthma.
0040-6376
328-332
Papadopoulos, N.G.
fac5aea9-7ee2-4579-90c8-e547b6a4bdf7
Stanciu, L.A.
0c4d1073-f1b5-4b4b-8aa0-90694922cd3e
Papi, A.
e64601e5-163a-416d-867d-c548aa80dd75
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Johnston, S.L.
f31c4376-1118-4eba-807d-9264c8df10a1
Papadopoulos, N.G.
fac5aea9-7ee2-4579-90c8-e547b6a4bdf7
Stanciu, L.A.
0c4d1073-f1b5-4b4b-8aa0-90694922cd3e
Papi, A.
e64601e5-163a-416d-867d-c548aa80dd75
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Johnston, S.L.
f31c4376-1118-4eba-807d-9264c8df10a1

Papadopoulos, N.G., Stanciu, L.A., Papi, A., Holgate, S.T. and Johnston, S.L. (2002) A defective type 1 response to rhinovirus in atopic asthma. Thorax, 57 (4), 328-332.

Record type: Article

Abstract

Background: Rhinoviruses (RVs) are the most frequent precipitants of the common cold and asthma exacerbations, but little is known about the immune response to these viruses and its potential implications in the pathogenesis of asthma.
Methods: Peripheral blood mononuclear cells (PBMC) from patients with atopic asthma and normal subjects were exposed to live or inactivated RV preparations. Levels of interferon (IFN)? and interleukins IL-12, IL-10, IL-4, IL-5 and IL-13 were evaluated in the culture supernatants with specific immunoassays.
Results: Exposure of PBMC to RVs induced the production of IFN?, IL-12, IL-10, and IL-13. Cells from asthmatic subjects produced significantly lower levels of IFN? and IL-12 and higher levels of IL-10 than normal subjects. IL-4 was induced only in the asthmatic group, while the IFN?/IL-4 ratio was more than three times lower in the asthmatic group.
Conclusions: This evidence suggests that the immune response to RVs is not uniquely of a type 1 phenotype, as previously suggested. The type 1 response is defective in atopic asthmatic individuals, with a shift towards a type 2 phenotype in a way similar, but not identical, to their aberrant response to allergens. A defective type 1 immune response to RVs may be implicated in the pathogenesis of virus induced exacerbations of asthma.

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Published date: 2002

Identifiers

Local EPrints ID: 27309
URI: http://eprints.soton.ac.uk/id/eprint/27309
ISSN: 0040-6376
PURE UUID: f0615163-a273-449d-a946-9bf282e35358

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Date deposited: 26 Apr 2006
Last modified: 09 Jan 2022 08:43

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Contributors

Author: N.G. Papadopoulos
Author: L.A. Stanciu
Author: A. Papi
Author: S.T. Holgate
Author: S.L. Johnston

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