Basic fibroblast growth factor in asthma: measurement in bronchoalveolar lavage fluid basally and following allergen challenge
Basic fibroblast growth factor in asthma: measurement in bronchoalveolar lavage fluid basally and following allergen challenge
Airway remodeling in asthma refers to a collection of chronic structural changes including subepithelial fibrosis, airway smooth muscle hypertrophy/hyperplasia, and possibly angiogenesis. The mechanisms leading to remodeling are not well defined. One molecule of possible relevance is basic fibroblast growth factor (bFGF), which is a potent mitogen for fibro-blasts, airway smooth muscle cells, and endothelial cells. To test the hypothesis that bFGF expression is increased in asthma, we measured levels of the growth factor in bronchoalveolar lavage (BAL) fluid. Basally, BAL fluid bFGF concentrations were significantly higher in subjects with atopic asthma than in control subjects without asthma (median 0.22 vs 0.06 pg/mL, P = .003). The effect of acute allergen exposure was examined with a segmental bronchoprovocation model in a separate group of subjects with atopic asthma. Ten minutes after segmental bronchoprovocation there was a 5-fold increase in bFGF levels in BAL fluid recovered from allergen-challenged sites compared with control saline-challenged sites (1.52 vs 0.30 pg/mL, P < .002). We conclude that basal levels of BAL fluid bFGF are increased in atopic asthma and that a further increase occurs in response to acute allergen exposure. These findings lend support to the hypothesis that bFGF is implicated in airway remodeling in asthma.
airway remodeling, basic fibroblast growth factor, bronchoalveolar lavage, bronchoscopy, fibrosis
384-387
Redington, Anthony E.
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Roche, William R.
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Madden, Jacqueline
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Frew, Anthony J.
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Djukanovic, Ratko
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Holgate, Stephen T.
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Howarth, Peter H.
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February 2001
Redington, Anthony E.
d61be7d5-850e-4d5c-a078-5603af44371e
Roche, William R.
a5135b2d-cab5-481b-887a-78611fa00bff
Madden, Jacqueline
0771e352-d432-41ea-8a7e-4704c1efca46
Frew, Anthony J.
4887b766-67c6-4d69-940d-4c06c0890b76
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Howarth, Peter H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Redington, Anthony E., Roche, William R., Madden, Jacqueline, Frew, Anthony J., Djukanovic, Ratko, Holgate, Stephen T. and Howarth, Peter H.
(2001)
Basic fibroblast growth factor in asthma: measurement in bronchoalveolar lavage fluid basally and following allergen challenge.
Journal of Allergy and Clinical Immunology, 107 (2), .
Abstract
Airway remodeling in asthma refers to a collection of chronic structural changes including subepithelial fibrosis, airway smooth muscle hypertrophy/hyperplasia, and possibly angiogenesis. The mechanisms leading to remodeling are not well defined. One molecule of possible relevance is basic fibroblast growth factor (bFGF), which is a potent mitogen for fibro-blasts, airway smooth muscle cells, and endothelial cells. To test the hypothesis that bFGF expression is increased in asthma, we measured levels of the growth factor in bronchoalveolar lavage (BAL) fluid. Basally, BAL fluid bFGF concentrations were significantly higher in subjects with atopic asthma than in control subjects without asthma (median 0.22 vs 0.06 pg/mL, P = .003). The effect of acute allergen exposure was examined with a segmental bronchoprovocation model in a separate group of subjects with atopic asthma. Ten minutes after segmental bronchoprovocation there was a 5-fold increase in bFGF levels in BAL fluid recovered from allergen-challenged sites compared with control saline-challenged sites (1.52 vs 0.30 pg/mL, P < .002). We conclude that basal levels of BAL fluid bFGF are increased in atopic asthma and that a further increase occurs in response to acute allergen exposure. These findings lend support to the hypothesis that bFGF is implicated in airway remodeling in asthma.
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More information
Submitted date: 28 January 2000
Published date: February 2001
Keywords:
airway remodeling, basic fibroblast growth factor, bronchoalveolar lavage, bronchoscopy, fibrosis
Organisations:
Infection Inflammation & Immunity
Identifiers
Local EPrints ID: 27350
URI: http://eprints.soton.ac.uk/id/eprint/27350
ISSN: 0091-6749
PURE UUID: 106faef6-aa20-4ecd-b280-785fc0a7eab3
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Date deposited: 28 Apr 2006
Last modified: 03 Aug 2022 01:32
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Contributors
Author:
Anthony E. Redington
Author:
William R. Roche
Author:
Jacqueline Madden
Author:
Anthony J. Frew
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