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Remodelling of cerebrospinal fluid lipoproteins after subarachnoid hemorrhage

Remodelling of cerebrospinal fluid lipoproteins after subarachnoid hemorrhage
Remodelling of cerebrospinal fluid lipoproteins after subarachnoid hemorrhage
Lipoprotein particles (Lps) in normal human cerebrospinal fluid (CSF) are distinct from those found in plasma and include unique apolipoprotein E (apoE indicates protein; APOE, gene) containing lipoproteins rarely seen in human plasma. Less favourable neurological recovery after subarachnoid hemorrhage (SAH) has been observed in patients who posses the APOE 4 allele raising the possibility that apoE influences neuronal survival after brain injury. We analysed Lps from control and SAH CSF testing the hypotheses that following brain injury CSF Lps undergo remodelling and apoE containing Lps are selectively depleted from brain injury CSF. Lipoproteins were fractionated using CSF from six control pools and six patients with SAH on a sepharose 6HR 10/30 size exclusion column. Fractions were assayed for total cholesterol (TC), free cholesterol (FC), phospholipid, triglyceride (TG), apoE, apolipoprotein B (apoB), and apolipoprotein AI (apoAI). Compared to control CSF there were significant (P<0.05) increases in TC, FC, TG, and apoAI in SAH CSF. Plasma sized apoB-containing lipoproteins and a very small apoAI-containing Lps were identified in the SAH CSF, which were not present in controls. However, despite the release of plasma lipoproteins into the subarachnoid space, there was no significant increase in CSF apoE. These data provide novel indirect evidence suggesting that after SAH CSF Lps undergo remodelling and apoE containing Lps are selectively reduced in brain injury CSF. The remodelling of CSF Lps and selective reduction of apoE containing lipoproteins may reflect an important response of the human brain to injury.
cerebrospinal fluid, lipoproteins, subarachnoid hemorrhage, brain injury
0021-9150
141-146
Kay, A.D.
818b43cc-0c10-4504-9ee5-847844d558e4
Day, S.P.
28f85461-ad26-4557-88bc-08ba44c3fbed
Nicoll, J.A.
88c0685f-000e-4eb7-8f72-f36b4985e8ed
Packard, C.J.
b1585baf-c1a7-4e97-b121-d6903a012050
Caslake, M.J.
2f6f6ea6-d99a-45d1-b543-367af7539054
Kay, A.D.
818b43cc-0c10-4504-9ee5-847844d558e4
Day, S.P.
28f85461-ad26-4557-88bc-08ba44c3fbed
Nicoll, J.A.
88c0685f-000e-4eb7-8f72-f36b4985e8ed
Packard, C.J.
b1585baf-c1a7-4e97-b121-d6903a012050
Caslake, M.J.
2f6f6ea6-d99a-45d1-b543-367af7539054

Kay, A.D., Day, S.P., Nicoll, J.A., Packard, C.J. and Caslake, M.J. (2003) Remodelling of cerebrospinal fluid lipoproteins after subarachnoid hemorrhage. Atherosclerosis, 170 (1), 141-146. (doi:10.1016/S0021-9150(03)00249-1).

Record type: Article

Abstract

Lipoprotein particles (Lps) in normal human cerebrospinal fluid (CSF) are distinct from those found in plasma and include unique apolipoprotein E (apoE indicates protein; APOE, gene) containing lipoproteins rarely seen in human plasma. Less favourable neurological recovery after subarachnoid hemorrhage (SAH) has been observed in patients who posses the APOE 4 allele raising the possibility that apoE influences neuronal survival after brain injury. We analysed Lps from control and SAH CSF testing the hypotheses that following brain injury CSF Lps undergo remodelling and apoE containing Lps are selectively depleted from brain injury CSF. Lipoproteins were fractionated using CSF from six control pools and six patients with SAH on a sepharose 6HR 10/30 size exclusion column. Fractions were assayed for total cholesterol (TC), free cholesterol (FC), phospholipid, triglyceride (TG), apoE, apolipoprotein B (apoB), and apolipoprotein AI (apoAI). Compared to control CSF there were significant (P<0.05) increases in TC, FC, TG, and apoAI in SAH CSF. Plasma sized apoB-containing lipoproteins and a very small apoAI-containing Lps were identified in the SAH CSF, which were not present in controls. However, despite the release of plasma lipoproteins into the subarachnoid space, there was no significant increase in CSF apoE. These data provide novel indirect evidence suggesting that after SAH CSF Lps undergo remodelling and apoE containing Lps are selectively reduced in brain injury CSF. The remodelling of CSF Lps and selective reduction of apoE containing lipoproteins may reflect an important response of the human brain to injury.

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More information

Published date: September 2003
Keywords: cerebrospinal fluid, lipoproteins, subarachnoid hemorrhage, brain injury

Identifiers

Local EPrints ID: 27614
URI: http://eprints.soton.ac.uk/id/eprint/27614
ISSN: 0021-9150
PURE UUID: 62b42d36-dee4-4edf-ae69-dae13fdafd1f
ORCID for J.A. Nicoll: ORCID iD orcid.org/0000-0002-9444-7246

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Date deposited: 27 Apr 2006
Last modified: 16 Mar 2024 03:26

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Contributors

Author: A.D. Kay
Author: S.P. Day
Author: J.A. Nicoll ORCID iD
Author: C.J. Packard
Author: M.J. Caslake

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