Synthesis and evaluation of fluorescent probes for the detection of calpain activity
Mittoo, Stifun, Sundstrom, Lars E. and Bradley, Mark (2003) Synthesis and evaluation of fluorescent probes for the detection of calpain activity. Analytical Biochemistry, 319, (2), 234-238. (doi:10.1016/S0003-2697(03)00324-5).
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Two new probes for the detection of calpain I activity based on fluorescence resonance energy transfer technology have been synthesized and evaluated. The probes incorporated the cleavage site present in ?-spectrin, a naturally occurring substrate of calpain I. The design of the internally quenched substrates is such that the calpain-sensitive bond of the peptides (between the Tyr-Gly residues) is located centrally between the donor and the quencher chromophores. The calpain assay protocol is capable of detecting enzymatic activity in the nanomolar region.
|Digital Object Identifier (DOI):||doi:10.1016/S0003-2697(03)00324-5|
|Keywords:||calpain i, fret, fluorescence, proteolytic activity|
|Subjects:||T Technology > TP Chemical technology
R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Clinical Neurosciences
|Date Deposited:||28 Apr 2006|
|Last Modified:||31 Mar 2016 11:52|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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