Synthesis and evaluation of fluorescent probes for the detection of calpain activity


Mittoo, Stifun, Sundstrom, Lars E. and Bradley, Mark (2003) Synthesis and evaluation of fluorescent probes for the detection of calpain activity. Analytical Biochemistry, 319, (2), 234-238. (doi:10.1016/S0003-2697(03)00324-5).

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Description/Abstract

Two new probes for the detection of calpain I activity based on fluorescence resonance energy transfer technology have been synthesized and evaluated. The probes incorporated the cleavage site present in α-spectrin, a naturally occurring substrate of calpain I. The design of the internally quenched substrates is such that the calpain-sensitive bond of the peptides (between the Tyr-Gly residues) is located centrally between the donor and the quencher chromophores. The calpain assay protocol is capable of detecting enzymatic activity in the nanomolar region.

Item Type: Article
ISSNs: 0003-2697 (print)
Related URLs:
Keywords: calpain i, fret, fluorescence, proteolytic activity
Subjects: T Technology > TP Chemical technology
R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
Divisions: University Structure - Pre August 2011 > School of Medicine > Clinical Neurosciences
ePrint ID: 27662
Date Deposited: 28 Apr 2006
Last Modified: 27 Mar 2014 18:16
URI: http://eprints.soton.ac.uk/id/eprint/27662

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