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Time window and pharmacological characterisation of kainate-mediated preconditioning in organotypic rat hippocampal slice cultures

Time window and pharmacological characterisation of kainate-mediated preconditioning in organotypic rat hippocampal slice cultures
Time window and pharmacological characterisation of kainate-mediated preconditioning in organotypic rat hippocampal slice cultures
Tolerance to normally neurotoxic insults can be induced by prior a preconditioning exposure to a sublethal insult. Kainate toxicity can be attenuated by prior exposure to very low concentrations of kainate both in vivo and in vitro. Using organotypic hippocampal slice cultures from rats we have shown that 5 ?M kainate induces a selective lesion in the CA3 region and this can be significantly attenuated by 1 ?M kainate administered 1–5 days earlier. The time window for this effect was affected by the length of time in culture, and preconditioning was blocked by NBQX but not the selective AMPA receptor antagonist GYKI53655. These data demonstrate a role for kainate receptors in preconditioning for the first time and show that organotypic cultures can be used as a model to investigate long-term preconditioning mechanisms.
kainic acid, excitotoxicity, preconditioning, organotypic culture, hippocampus
0304-3940
365-368
Nvue, Rose
a7acaa52-d1ea-495a-8979-53a8798d91aa
Gorianov, Vitaly
e2d8b62b-fe72-45de-b469-309e6c9f3293
Best, Nick
8aa6cc54-07ed-45e4-8459-9b0cef041e4c
Sundstrom, Lars E.
bb62018d-0157-4274-a865-448ed12934bd
Pringle, Ashley K.
6339ed95-c491-43a8-b2fb-2384466dc80d
Nvue, Rose
a7acaa52-d1ea-495a-8979-53a8798d91aa
Gorianov, Vitaly
e2d8b62b-fe72-45de-b469-309e6c9f3293
Best, Nick
8aa6cc54-07ed-45e4-8459-9b0cef041e4c
Sundstrom, Lars E.
bb62018d-0157-4274-a865-448ed12934bd
Pringle, Ashley K.
6339ed95-c491-43a8-b2fb-2384466dc80d

Nvue, Rose, Gorianov, Vitaly, Best, Nick, Sundstrom, Lars E. and Pringle, Ashley K. (2004) Time window and pharmacological characterisation of kainate-mediated preconditioning in organotypic rat hippocampal slice cultures. Neuroscience Letters, 367 (3), 365-368. (doi:10.1016/j.neulet.2004.06.033).

Record type: Article

Abstract

Tolerance to normally neurotoxic insults can be induced by prior a preconditioning exposure to a sublethal insult. Kainate toxicity can be attenuated by prior exposure to very low concentrations of kainate both in vivo and in vitro. Using organotypic hippocampal slice cultures from rats we have shown that 5 ?M kainate induces a selective lesion in the CA3 region and this can be significantly attenuated by 1 ?M kainate administered 1–5 days earlier. The time window for this effect was affected by the length of time in culture, and preconditioning was blocked by NBQX but not the selective AMPA receptor antagonist GYKI53655. These data demonstrate a role for kainate receptors in preconditioning for the first time and show that organotypic cultures can be used as a model to investigate long-term preconditioning mechanisms.

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More information

Published date: 2004
Keywords: kainic acid, excitotoxicity, preconditioning, organotypic culture, hippocampus

Identifiers

Local EPrints ID: 27678
URI: http://eprints.soton.ac.uk/id/eprint/27678
ISSN: 0304-3940
PURE UUID: ae0cecf7-4b9a-480d-aa33-6e4888403c78
ORCID for Ashley K. Pringle: ORCID iD orcid.org/0000-0003-2421-4380

Catalogue record

Date deposited: 25 Apr 2006
Last modified: 16 Mar 2024 02:48

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Contributors

Author: Rose Nvue
Author: Vitaly Gorianov
Author: Nick Best
Author: Lars E. Sundstrom

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