Characterisation of a novel class of polyamine-based neuroprotective compounds


Pringle, Ashley K., Morrison III, Barclay, Bradley, Mark, Iannotti, Fausto and Sundstrom, Lars E. (2003) Characterisation of a novel class of polyamine-based neuroprotective compounds. Naunyn-Schmiedeberg's Archives of Pharmacology, 368, (3), 216-224. (doi:10.1007/s00210-003-0778-4).

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Description/Abstract

Prolonged cerebral ischaemia initiates complex intra- and inter-cellular signalling cascades ultimately resulting in neuronal death. Well-characterised mediators of ischaemic cell death are glutamate, free radicals and nitric oxide. Many drugs that block these mechanisms are neuroprotective in vitro, but have unfavourable side-effect profiles in man. We have recently demonstrated that the compound L-arginyl-3,4-spermidine (L-Arg3,4) is neuroprotective in vitro through an interaction with several of these mechanisms, and prevents ischaemic neurodegeneration in vivo with no gross side effects. In this study, we have used solid-phase combinatorial chemistry, to synthesise a number of analogues of L-Arg3,4, and investigate the structure-activity relationship using an in vitro, organotypic hippocampal slice culture model of cerebral ischaemia. A number of molecular features were identified which were essential for the neuroprotective activity including the requirement for a positive charge and an amino acid in the L-configuration. Relatively minor alterations to both the terminal arginine and polyamine moieties significantly attenuated neuroprotective efficacy. Our data implies that these compounds are neuroprotective through a currently undefined mechanism rather than non-specific ionic interactions described previously for other polyamine-containing compounds.

Item Type: Article
ISSNs: 0028-1298 (print)
Related URLs:
Keywords: organotypic culture, hippocampus, hypoxia, polyamine, neuroprotection
Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions: University Structure - Pre August 2011 > School of Medicine > Clinical Neurosciences
ePrint ID: 27690
Date Deposited: 28 Apr 2006
Last Modified: 27 Mar 2014 18:16
Contact Email Address: akp1@soton.ac.uk
URI: http://eprints.soton.ac.uk/id/eprint/27690

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