The expression of the developmentally regulated proto-oncogene Pax-3 is modulated by N-Myc


Harris, Robert G., White, Edward, Phillips, Emma S. and Lillycrop, Karen A. (2002) The expression of the developmentally regulated proto-oncogene Pax-3 is modulated by N-Myc. Journal of Biological Chemistry, 277, (38), 34815-34825. (doi:10.1074/jbc.M109609200).

Download

[img]
Preview
PDF
Download (449Kb)
Original Publication URL: http://dx.doi.org/10.1074/jbc.M109609200

Description/Abstract

N-Myc is a member of the Myc family of transcription factors that have been shown to play a pivotal role in cell proliferation and differentiation. In this report, we have investigated the relationship between N-Myc and the developmental control gene Pax-3. Using transient transfection assays, we show that the Pax-3 promoter is activated by both N-Myc-Max and c-Myc-Max. Moreover, we show that Myc regulation of Pax-3 promoter activity is dependent upon a noncanonical E box site in the 5' promoter region of Pax-3. In addition, we show that ectopic expression of both N-Myc and c-Myc leads to increased expression of Pax-3 mRNA. Furthermore, we show that Pax-3 mRNA expression is cell cycle-regulated and that the 5' promoter region of Pax-3 (bp - 1578 to +56) can direct cell cycle-dependent gene expression with kinetics similar to that of the endogenous transcript. Site-directed mutagenesis of the E box site within the Pax-3 promoter significantly altered the pattern of expression through the cell cycle. These results suggest that the Myc family of transcription factors may modulate Pax-3 expression in vivo.

Item Type: Article
ISSNs: 0021-9258 (print)
Related URLs:
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Divisions: University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 28824
Date Deposited: 08 May 2006
Last Modified: 27 Mar 2014 18:17
URI: http://eprints.soton.ac.uk/id/eprint/28824

Actions (login required)

View Item View Item

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics