The expression of the developmentally regulated proto-oncogene Pax-3 is modulated by N-Myc
Harris, Robert G., White, Edward, Phillips, Emma S. and Lillycrop, Karen A. (2002) The expression of the developmentally regulated proto-oncogene Pax-3 is modulated by N-Myc. Journal of Biological Chemistry, 277, (38), 34815-34825. (doi:10.1074/jbc.M109609200).
N-Myc is a member of the Myc family of transcription factors that have been shown to play a pivotal role in cell proliferation and differentiation. In this report, we have investigated the relationship between N-Myc and the developmental control gene Pax-3. Using transient transfection assays, we show that the Pax-3 promoter is activated by both N-Myc-Max and c-Myc-Max. Moreover, we show that Myc regulation of Pax-3 promoter activity is dependent upon a noncanonical E box site in the 5' promoter region of Pax-3. In addition, we show that ectopic expression of both N-Myc and c-Myc leads to increased expression of Pax-3 mRNA. Furthermore, we show that Pax-3 mRNA expression is cell cycle-regulated and that the 5' promoter region of Pax-3 (bp - 1578 to +56) can direct cell cycle-dependent gene expression with kinetics similar to that of the endogenous transcript. Site-directed mutagenesis of the E box site within the Pax-3 promoter significantly altered the pattern of expression through the cell cycle. These results suggest that the Myc family of transcription factors may modulate Pax-3 expression in vivo.
|Subjects:||Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
|Divisions:||University Structure - Pre August 2011 > School of Biological Sciences
|Date Deposited:||08 May 2006|
|Last Modified:||28 Jun 2012 10:12|
|Contributors:||Harris, Robert G. (Author)
White, Edward (Author)
Phillips, Emma S. (Author)
Lillycrop, Karen A. (Author)
|Date:||2 July 2002|
|Contact Email Address:||KAL@soton.ac.uk|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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