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Biosensors for unattended cost-effective and continuous monitoring of environmental pollution: Automated water analyser computer supported system (AWACSS) and river analyser (RIANA)

Biosensors for unattended cost-effective and continuous monitoring of environmental pollution: Automated water analyser computer supported system (AWACSS) and river analyser (RIANA)
Biosensors for unattended cost-effective and continuous monitoring of environmental pollution: Automated water analyser computer supported system (AWACSS) and river analyser (RIANA)
This work describes our recent progress and achievements in the field of fully automated biosensors (Automated Water Analyser Computer Supported System (AWACSS) and River Analyser (RIANA)) for unattended, cost-effective and continuous monitoring of environmental pollution. We report on ultra-sensitive immunoassays for the hormones progesterone, testosterone and estrone and the pesticides propanil and isoproturon as examples of the outstanding progress made on biosensors in the field of environmental monitoring and water analysis. Most of the bio-active organic pollutants (estrone, progesterone, propanil and isoproturon) were detected at levels as low as 1.0pgm/L or even below.

In fact, the reported limits of detection (LOD) were between 0.2 and 6.0pgm/L. For the first time, commercially available derivatives and antibodies were incorporated into immunoassays (progesterone and testosterone) for fully automated biosensors. To verify the assay performance for quantifying testosterone, progesterone, and isoproturon in real-world samples using our immunosensors, we spiked river and drinking water at six different levels from 0.9pgm/L to 90ngm/L. Nearly all recovery rates could be obtained between 70 and 120% as the AOAC International recommends it chiefly for water analysis.
automated water analyser computer supported system, river analyser (riana), optical immunosensor, environmental analysis, bio-active organic pollutants, continuous monitoring
0306-7319
837-852
Tschmelak, Jens
aa5c4cb8-42fd-496e-a0ad-bbce99e2b568
Proll, Guenther
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Riedt, Johannes
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Kaiser, Joachim
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Kraemmer, Peter
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Bárzaga, Luis
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Wilkinson, James
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Hua, Ping
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Hole, J. Patrick
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Nudd, Richard
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Jackson, Michael
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Abuknesha, Ram
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Barceló, Damià
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Rodriguez-Mozaz, Sara
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López de Alda, Maria J.
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Sacher, Frank
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Stien, Jan
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Slobodník, Jaroslav
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Oswald, Peter
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Kozmenko, Helena
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Korenková, Eva
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Tóthová, Lívia
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Krascsenits, Zoltan
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Gauglitz, Guenter
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Tschmelak, Jens
aa5c4cb8-42fd-496e-a0ad-bbce99e2b568
Proll, Guenther
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Riedt, Johannes
6a4f9744-83ff-4970-b323-b4f09ade39c4
Kaiser, Joachim
0e487776-21c4-44c9-a34d-bfa3cc6bd1cd
Kraemmer, Peter
aedb03c3-1807-4e28-92d6-42e2cb386984
Bárzaga, Luis
96470766-d13c-4d35-811d-ab29c64e012f
Wilkinson, James
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Hua, Ping
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Hole, J. Patrick
0ce2d748-7b31-4ada-bad0-e9af21cdb192
Nudd, Richard
a52c6c1b-2ba7-44c3-b330-8d8e3e0e207e
Jackson, Michael
6ebc220f-eda6-497d-b761-0d053ccfd220
Abuknesha, Ram
dad4314b-6eb6-478e-8d9a-00cfc50bd0a1
Barceló, Damià
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Rodriguez-Mozaz, Sara
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López de Alda, Maria J.
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Sacher, Frank
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Stien, Jan
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Slobodník, Jaroslav
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Oswald, Peter
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Kozmenko, Helena
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Korenková, Eva
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Tóthová, Lívia
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Krascsenits, Zoltan
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Gauglitz, Guenter
c7a244e5-e7dc-4dce-a9fd-c83945597956

Tschmelak, Jens, Proll, Guenther, Riedt, Johannes, Kaiser, Joachim, Kraemmer, Peter, Bárzaga, Luis, Wilkinson, James, Hua, Ping, Hole, J. Patrick, Nudd, Richard, Jackson, Michael, Abuknesha, Ram, Barceló, Damià, Rodriguez-Mozaz, Sara, López de Alda, Maria J., Sacher, Frank, Stien, Jan, Slobodník, Jaroslav, Oswald, Peter, Kozmenko, Helena, Korenková, Eva, Tóthová, Lívia, Krascsenits, Zoltan and Gauglitz, Guenter (2005) Biosensors for unattended cost-effective and continuous monitoring of environmental pollution: Automated water analyser computer supported system (AWACSS) and river analyser (RIANA). International Journal of Environmental Analytical Chemistry, 85 (12-13), 837-852. (doi:10.1080/03067310500149619).

Record type: Article

Abstract

This work describes our recent progress and achievements in the field of fully automated biosensors (Automated Water Analyser Computer Supported System (AWACSS) and River Analyser (RIANA)) for unattended, cost-effective and continuous monitoring of environmental pollution. We report on ultra-sensitive immunoassays for the hormones progesterone, testosterone and estrone and the pesticides propanil and isoproturon as examples of the outstanding progress made on biosensors in the field of environmental monitoring and water analysis. Most of the bio-active organic pollutants (estrone, progesterone, propanil and isoproturon) were detected at levels as low as 1.0pgm/L or even below.

In fact, the reported limits of detection (LOD) were between 0.2 and 6.0pgm/L. For the first time, commercially available derivatives and antibodies were incorporated into immunoassays (progesterone and testosterone) for fully automated biosensors. To verify the assay performance for quantifying testosterone, progesterone, and isoproturon in real-world samples using our immunosensors, we spiked river and drinking water at six different levels from 0.9pgm/L to 90ngm/L. Nearly all recovery rates could be obtained between 70 and 120% as the AOAC International recommends it chiefly for water analysis.

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More information

Published date: 2005
Keywords: automated water analyser computer supported system, river analyser (riana), optical immunosensor, environmental analysis, bio-active organic pollutants, continuous monitoring

Identifiers

Local EPrints ID: 30197
URI: http://eprints.soton.ac.uk/id/eprint/30197
ISSN: 0306-7319
PURE UUID: a1b0a1ff-8170-4b54-a883-9ffe379035b3
ORCID for James Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 11 May 2006
Last modified: 16 Mar 2024 02:33

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Contributors

Author: Jens Tschmelak
Author: Guenther Proll
Author: Johannes Riedt
Author: Joachim Kaiser
Author: Peter Kraemmer
Author: Luis Bárzaga
Author: James Wilkinson ORCID iD
Author: Ping Hua
Author: J. Patrick Hole
Author: Richard Nudd
Author: Michael Jackson
Author: Ram Abuknesha
Author: Damià Barceló
Author: Sara Rodriguez-Mozaz
Author: Maria J. López de Alda
Author: Frank Sacher
Author: Jan Stien
Author: Jaroslav Slobodník
Author: Peter Oswald
Author: Helena Kozmenko
Author: Eva Korenková
Author: Lívia Tóthová
Author: Zoltan Krascsenits
Author: Guenter Gauglitz

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