Contrast agent-free cell sonoporation using a continuous-flow microfluidic device
Carugo, Dario, Ankrett, Dyan N., Glynne-Jones, Peter, Capretto, Lorenzo, Boltryk, Rosemary J., Townsend, Paul A., Zhang, Xunli and Hill, Martyn (2011) Contrast agent-free cell sonoporation using a continuous-flow microfluidic device. In, The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS ), Seattle, US, 02 Oct 2011 - 06 Oct 2012 .
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Ultrasound (US) has been utilised to induce reversible poration of cell membranes (sonoporation) in order to facilitate the trans-membrane delivery of pharmaceutical agents. Sonoporation is conventionally performed in the presence of microbubble contrast agents (CA). However, challenges remain in achieving controlled sonoporation due to CA cavitational effects, which can significantly reduce cell viability. Therefore CA-free sonoporation represents a “gentle” methodology for facilitating the delivery of pharmaceutical agents while maintaining high cell viability. We report facilitated uptake of doxorubicin and apigenin by CA free sonoporation of suspended cardiomyocytes by generating an ultrasonic standing wave within an easy to-use continuous-flow microfluidic device
|Item Type:||Conference or Workshop Item (Paper)|
|Subjects:||R Medicine > R Medicine (General)
T Technology > T Technology (General)
|Divisions:||Faculty of Engineering and the Environment > Engineering Sciences > Bioengineering Research Group
Faculty of Engineering and the Environment > Engineering Sciences > Electro-Mechanical Research Group
Faculty of Medicine > Cancer Sciences
|Date Deposited:||08 Mar 2012 11:44|
|Last Modified:||08 Mar 2012 11:44|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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