Contrast agent-free cell sonoporation using a continuous-flow microfluidic device


Carugo, Dario, Ankrett, Dyan N., Glynne-Jones, Peter, Capretto, Lorenzo, Boltryk, Rosemary J., Townsend, Paul A., Zhang, Xunli and Hill, Martyn (2011) Contrast agent-free cell sonoporation using a continuous-flow microfluidic device. In, The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS ), Seattle, US, 02 Oct 2011 - 06 Oct 2012 .

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Description/Abstract

Ultrasound (US) has been utilised to induce reversible poration of cell membranes (sonoporation) in order to facilitate the trans-membrane delivery of pharmaceutical agents. Sonoporation is conventionally performed in the presence of microbubble contrast agents (CA). However, challenges remain in achieving controlled sonoporation due to CA cavitational effects, which can significantly reduce cell viability. Therefore CA-free sonoporation represents a “gentle” methodology for facilitating the delivery of pharmaceutical agents while maintaining high cell viability. We report facilitated uptake of doxorubicin and apigenin by CA free sonoporation of suspended cardiomyocytes by generating an ultrasonic standing wave within an easy to-use continuous-flow microfluidic device

Item Type: Conference or Workshop Item (Paper)
Related URLs:
Subjects: R Medicine > R Medicine (General)
T Technology > T Technology (General)
Divisions: Faculty of Engineering and the Environment > Engineering Sciences > Bioengineering Research Group
Faculty of Engineering and the Environment > Engineering Sciences > Electro-Mechanical Research Group
Faculty of Medicine > Cancer Sciences
ePrint ID: 333944
Date Deposited: 08 Mar 2012 11:44
Last Modified: 14 Apr 2014 11:37
Projects:
Simulated Cardiac Cell Trauma using Gentle Ultrasonic Streaming
Funded by: EPSRC (EP/H01392X/1)
Led by: Rosemary June Boltryk
26 April 2010 to 25 April 2011
URI: http://eprints.soton.ac.uk/id/eprint/333944

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