Up-regulation of p21WAF1/CIP1 in psoriasis and after the application of irritants and tape stripping

Healy, Eugene, Reynolds, Nicholas J., Smith, Martin D., Harrison, David, Doherty, Elaine, Campbell, Christine and Rees, Jonathan L. (1995) Up-regulation of p21WAF1/CIP1 in psoriasis and after the application of irritants and tape stripping. Journal of Investigative Dermatology, 105, (2), 274-279. (doi:10.1111/1523-1747.ep12318430). (PMID:7636313).


Full text not available from this repository.


p21WAF1/CIP1 is a nucleoprotein that was initially characterized by its ability to be regulated transcriptionally by p53 and by its ability to mediate growth arrest by binding to cyclin-dependent kinases. Although p21WAF1/CIP1 is thought to mediate the effects of p53 in causing growth arrest, p21WAF1/CIP1 is also regulated in a p53-independent manner, e.g., during terminal differentiation of some cell lines. Growth factors including epidermal growth factor also induce p21WAF1/CIP1 through p53-independent pathways. Because the epidermal growth factor signaling pathway is abnormal in psoriatic epidermis, we studied p21WAF1/CIP1 expression, using in situ hybridization and immunohistochemistry, in psoriasis. Both p21WAF1/CIP1 mRNA and protein were significantly elevated in untreated psoriatic plaques compared with uninvolved psoriatic skin (p < 0.0001), with the up-regulation of p21WAF1/CIP1 being predominantly suprabasal. This increase was accompanied by a small increase in p53 protein expression of uncertain significance. Furthermore, p21WAF1/CIP1 expression was induced in skin after sellotape stripping and by the application of agents, such as dithranol, that are capable of inducing hyperproliferation. The pattern of p21WAF1/CIP1 expression observed is consistent with a role in induction and maintenance of differentiation. Our experiments, however, cannot determine whether the abnormalities of p21WAF1/CIP1 epidermal expression in psoriasis and after insult are independent of changes in p53 expression.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1111/1523-1747.ep12318430
ISSNs: 0022-202X (print)
1523-1747 (electronic)
Keywords: differentiation, dithranol, p53, proliferation
Subjects: Q Science > QR Microbiology > QR180 Immunology
R Medicine > RL Dermatology
Divisions : Faculty of Medicine > Clinical and Experimental Sciences
ePrint ID: 334170
Accepted Date and Publication Date:
August 1995Published
Date Deposited: 14 Mar 2012 14:01
Last Modified: 31 Mar 2016 14:24
URI: http://eprints.soton.ac.uk/id/eprint/334170

Actions (login required)

View Item View Item