The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica
Martinez-Gomez, N. Cecilia, Palmer, Lauren D., Vivas, Eugenio, Roach, Peter L. and Downs, Diana M. (2011) The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica. Journal of Bacteriology, 193, (18), 4582-4587. (doi:10.1128/Jb.05325-11). (PMID:21724998).
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In Salmonella enterica, ThiI is a bifunctional enzyme required for the synthesis of both the 4-thiouridine modification in tRNA and the thiazole moiety of thiamine. In thiouridine biosynthesis, ThiI adenylates the tRNA uridine and transfers sulfur from a persulfide formed on the protein. The role of ThiI in thiazole synthesis is not yet well understood. Mutational analysis described herein identified ThiI residues required for thiouridine synthesis were not involved in thiazole biosynthesis. The data further showed that the C-terminal rhodanese domain of ThiI was sufficient for thiazole synthesis in vivo. Together these data support the conclusion that sulfur mobilization in thiazole synthesis is mechanistically distinct from that in 4-thiouridine synthesis, and suggest functional annotation of ThiI in genome sequences should be readdressed. Nutritional studies described herein identified an additional cysteine-dependent mechanism for sulfur mobilization to thiazole that did not require ThiI, IscS, SufS or glutathione. The later mechanism may provide insights into the chemistry used for sulfur mobilization to thiazole in organisms that do not utilize ThiI.
|Keywords:||thiazole synthesis, thiamine, ThiI, rhodanese domains, sulfur mobilization|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Divisions:||Faculty of Natural and Environmental Sciences > Chemistry
|Date Deposited:||30 Mar 2012 12:45|
|Last Modified:||30 Mar 2012 12:45|
|Contributors:||Martinez-Gomez, N. Cecilia (Author)
Palmer, Lauren D. (Author)
Vivas, Eugenio (Author)
Roach, Peter L. (Author)
Downs, Diana M. (Author)
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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