The self-orientation of mammalian cells in optical tweezers - the importance of the nucleus

Perney, Nicolas M.B., Horak, Peter, Hanley, Neil A. and Melvin, Tracy (2012) The self-orientation of mammalian cells in optical tweezers - the importance of the nucleus. Physical Biology, 9, (2), 024001. (doi:10.1088/1478-3975/9/2/024001). (PMID:22473086).


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Here we present the first evidence showing that eukaryotic cells can be stably trapped in a single focused Gaussian beam with an orientation that is defined by the nucleus. A mammalian eukaryotic cell (in suspension) is trapped and is re-oriented in the focus of a linearly polarized Gaussian beam with a waist of dimension smaller than the radius of the nucleus. The cell reaches a position relative to the focus that is dictated by the nucleus and nuclear components. Our studies illustrate that the force exerted by the optical tweezers at locations within the cell can be predicted theoretically; the data obtained in this way is consistent with the experimental observations.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1088/1478-3975/9/2/024001
ISSNs: 1478-3967 (print)
1478-3975 (electronic)
Related URLs:
Keywords: instrumentation and measurement, medical physics, biological physics
Subjects: Q Science > QC Physics
Q Science > QH Natural history > QH301 Biology
Divisions : Faculty of Physical Sciences and Engineering > Optoelectronics Research Centre
ePrint ID: 337291
Accepted Date and Publication Date:
3 April 2012Published
Date Deposited: 23 Apr 2012 13:41
Last Modified: 25 Jul 2016 11:56
Detecting cytosine methylation at the single DNA molecule level
Funded by: BBSRC (BB/I022791/1)
Led by: Tracy Melvin
9 January 2012 to 8 July 2015

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