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The self-orientation of mammalian cells in optical tweezers - the importance of the nucleus

The self-orientation of mammalian cells in optical tweezers - the importance of the nucleus
The self-orientation of mammalian cells in optical tweezers - the importance of the nucleus
Here we present the first evidence showing that eukaryotic cells can be stably trapped in a single focused Gaussian beam with an orientation that is defined by the nucleus. A mammalian eukaryotic cell (in suspension) is trapped and is re-oriented in the focus of a linearly polarized Gaussian beam with a waist of dimension smaller than the radius of the nucleus. The cell reaches a position relative to the focus that is dictated by the nucleus and nuclear components. Our studies illustrate that the force exerted by the optical tweezers at locations within the cell can be predicted theoretically; the data obtained in this way is consistent with the experimental observations.
instrumentation and measurement, medical physics, biological physics
1478-3967
24001
Perney, Nicolas M.B.
169bd490-31cb-4379-b25b-ae3e7880081b
Horak, Peter
520489b5-ccc7-4d29-bb30-c1e36436ea03
Hanley, Neil A.
bf03f7bb-f377-44fb-8344-0bb1ca8b2ef9
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Perney, Nicolas M.B.
169bd490-31cb-4379-b25b-ae3e7880081b
Horak, Peter
520489b5-ccc7-4d29-bb30-c1e36436ea03
Hanley, Neil A.
bf03f7bb-f377-44fb-8344-0bb1ca8b2ef9
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f

Perney, Nicolas M.B., Horak, Peter, Hanley, Neil A. and Melvin, Tracy (2012) The self-orientation of mammalian cells in optical tweezers - the importance of the nucleus. Physical Biology, 9 (2), 24001. (doi:10.1088/1478-3975/9/2/024001). (PMID:22473086)

Record type: Article

Abstract

Here we present the first evidence showing that eukaryotic cells can be stably trapped in a single focused Gaussian beam with an orientation that is defined by the nucleus. A mammalian eukaryotic cell (in suspension) is trapped and is re-oriented in the focus of a linearly polarized Gaussian beam with a waist of dimension smaller than the radius of the nucleus. The cell reaches a position relative to the focus that is dictated by the nucleus and nuclear components. Our studies illustrate that the force exerted by the optical tweezers at locations within the cell can be predicted theoretically; the data obtained in this way is consistent with the experimental observations.

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More information

Published date: 3 April 2012
Keywords: instrumentation and measurement, medical physics, biological physics
Organisations: Optoelectronics Research Centre

Identifiers

Local EPrints ID: 337291
URI: http://eprints.soton.ac.uk/id/eprint/337291
ISSN: 1478-3967
PURE UUID: 8236f2a9-4906-430f-a125-6505bc680ec0
ORCID for Peter Horak: ORCID iD orcid.org/0000-0002-8710-8764

Catalogue record

Date deposited: 23 Apr 2012 13:41
Last modified: 15 Mar 2024 03:13

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Contributors

Author: Nicolas M.B. Perney
Author: Peter Horak ORCID iD
Author: Neil A. Hanley
Author: Tracy Melvin

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