The self-orientation of mammalian cells in optical tweezers—the importance of the nucleus
Perney, Nicolas M.B., Horak, Peter, Hanley, Neil A. and Melvin, Tracy (2012) The self-orientation of mammalian cells in optical tweezers—the importance of the nucleus. Physical Biology, 9, (2), 024001. (doi:10.1088/1478-3975/9/2/024001). (PMID:22473086).
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Here we present the first evidence showing that eukaryotic cells can be stably trapped in a single focused Gaussian beam with an orientation that is defined by the nucleus. A mammalian eukaryotic cell (in suspension) is trapped and is re-oriented in the focus of a linearly polarized Gaussian beam with a waist of dimension smaller than the radius of the nucleus. The cell reaches a position relative to the focus that is dictated by the nucleus and nuclear components. Our studies illustrate that the force exerted by the optical tweezers at locations within the cell can be predicted theoretically; the data obtained in this way is consistent with the experimental observations.
|Digital Object Identifier (DOI):||doi:10.1088/1478-3975/9/2/024001|
|Keywords:||instrumentation and measurement, medical physics, biological physics|
|Subjects:||Q Science > QC Physics
Q Science > QH Natural history > QH301 Biology
|Divisions:||Faculty of Physical Sciences and Engineering > Optoelectronics Research Centre
|Date Deposited:||23 Apr 2012 13:41|
|Last Modified:||26 Sep 2014 12:13|
Detecting cytosine methylation at the single DNA molecule level
Funded by: BBSRC (BB/I022791/1)
Led by: Tracy Melvin
9 January 2012 to 8 July 2015
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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