Regulation of miR-200c by nuclear receptors PPARα, LRH-1 and SHP


Zhang, Yuxia, Yang, Zhihong, Whitby, Richard and Wang, Li (2011) Regulation of miR-200c by nuclear receptors PPARα, LRH-1 and SHP. Biochemical and Biophysical Research Communications, 416, (1-2), 135-139. (doi:10.1016/j.bbrc.2011.11.011). (PMID:22100809).

Download

Full text not available from this repository.

Description/Abstract

We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPARα and LRH-1 siRNAs. The expression of miR-200c was decreased by PPARα/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPARα and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.

Item Type: Article
ISSNs: 0006-291X (print)
1090-2104 (electronic)
Keywords: nuclear receptor, microRNA, transcription, cell migration
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Natural and Environmental Sciences > Chemistry
Faculty of Natural and Environmental Sciences > Chemistry > Molecular Assembly, Function & Structure
Faculty of Natural and Environmental Sciences > Chemistry > Synthetic Chemistry Group
ePrint ID: 337315
Date Deposited: 24 Apr 2012 09:27
Last Modified: 27 Mar 2014 20:20
URI: http://eprints.soton.ac.uk/id/eprint/337315

Actions (login required)

View Item View Item