Regulation of miR-200c by nuclear receptors PPAR?, LRH-1 and SHP
Regulation of miR-200c by nuclear receptors PPAR?, LRH-1 and SHP
We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPAR? and LRH-1 siRNAs. The expression of miR-200c was decreased by PPAR?/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPAR? and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.
nuclear receptor, microRNA, transcription, cell migration
135-139
Zhang, Yuxia
38d2c428-ad53-4907-8fbe-d26e495b79c4
Yang, Zhihong
e6e4733d-97db-4f87-aa0b-91db11901dc9
Whitby, Richard
45632236-ab00-4ad0-a02d-6209043e818b
Wang, Li
f54669eb-8e6b-43ea-a6df-47cda21d6950
9 December 2011
Zhang, Yuxia
38d2c428-ad53-4907-8fbe-d26e495b79c4
Yang, Zhihong
e6e4733d-97db-4f87-aa0b-91db11901dc9
Whitby, Richard
45632236-ab00-4ad0-a02d-6209043e818b
Wang, Li
f54669eb-8e6b-43ea-a6df-47cda21d6950
Zhang, Yuxia, Yang, Zhihong, Whitby, Richard and Wang, Li
(2011)
Regulation of miR-200c by nuclear receptors PPAR?, LRH-1 and SHP.
Biochemical and Biophysical Research Communications, 416 (1-2), .
(doi:10.1016/j.bbrc.2011.11.011).
(PMID:22100809)
Abstract
We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPAR? and LRH-1 siRNAs. The expression of miR-200c was decreased by PPAR?/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPAR? and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.
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Published date: 9 December 2011
Keywords:
nuclear receptor, microRNA, transcription, cell migration
Organisations:
Organic Chemistry: Synthesis, Catalysis and Flow, Chemistry
Identifiers
Local EPrints ID: 337315
URI: http://eprints.soton.ac.uk/id/eprint/337315
ISSN: 0006-291X
PURE UUID: 4e14f7bf-553f-4b18-8f75-31b197617ca5
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Date deposited: 24 Apr 2012 09:27
Last modified: 15 Mar 2024 02:34
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Contributors
Author:
Yuxia Zhang
Author:
Zhihong Yang
Author:
Li Wang
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