Regulation of miR-200c by nuclear receptors PPAR?, LRH-1 and SHP


Zhang, Yuxia, Yang, Zhihong, Whitby, Richard and Wang, Li (2011) Regulation of miR-200c by nuclear receptors PPAR?, LRH-1 and SHP. Biochemical and Biophysical Research Communications, 416, (1-2), 135-139. (doi:10.1016/j.bbrc.2011.11.011). (PMID:22100809).

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Description/Abstract

We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPAR? and LRH-1 siRNAs. The expression of miR-200c was decreased by PPAR?/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPAR? and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.bbrc.2011.11.011
ISSNs: 0006-291X (print)
1090-2104 (electronic)
Keywords: nuclear receptor, microRNA, transcription, cell migration
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions : Faculty of Natural and Environmental Sciences > Chemistry
Faculty of Natural and Environmental Sciences > Chemistry > Organic Chemistry: Synthesis, Catalysis and Flow
Faculty of Natural and Environmental Sciences > Chemistry > Characterisation and Analytics
ePrint ID: 337315
Accepted Date and Publication Date:
Status
9 December 2011Published
Date Deposited: 24 Apr 2012 09:27
Last Modified: 31 Mar 2016 14:26
URI: http://eprints.soton.ac.uk/id/eprint/337315

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