Circulating NO pool: assessment of nitrite and nitroso species in blood and tissues

Rassaf, Tienush, Feelisch, Martin and Kelm, Malte (2004) Circulating NO pool: assessment of nitrite and nitroso species in blood and tissues. Free Radical Biology and Medicine, 36, (4), 413-422. (doi:10.1016/j.freeradbiomed.2003.11.011). (PMID:14975444).


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The formation of nitric oxide (NO) has been linked to many regulatory functions in mammalian cells. With the appreciation that NO-mediated nitrosation reactions are involved in cell signaling and pathology there is a need to elucidate and better characterize the different biochemical pathways of NO in vivo. Despite significant methodological advances over the years one major obstacle in assessing the significance of nitrosated species and other NO-related metabolites remains: their reliable measurement in complex biological matrices. In this review we briefly discuss the major routes of NO metabolism and transport in the mammalian circulation, considering plasma, red blood cell, and tissue compartments separately. In addition, we attempt to give a recommendation as to the most appropriate analytical technique and sample processing procedures for the reliable quantification of either species.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.freeradbiomed.2003.11.011
ISSNs: 0891-5849 (print)
Subjects: Q Science > QR Microbiology > QR180 Immunology
R Medicine > RC Internal medicine
Divisions : Faculty of Medicine > Clinical and Experimental Sciences
ePrint ID: 337848
Accepted Date and Publication Date:
15 February 2004Published
19 December 2003Made publicly available
Date Deposited: 22 Jun 2012 09:30
Last Modified: 31 Mar 2016 14:27

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