Nitric oxide modulates endotoxin-induced platelet-endothelial cell adhesion in intestinal venules
Cerwinka, Wolfgang H., Cooper, Dianne, Krieglstein, Christian F., Feelisch, Martin and Granger, D. Neil (2002) Nitric oxide modulates endotoxin-induced platelet-endothelial cell adhesion in intestinal venules. American Journal of Physiology. Heart and Circulatory Physiology, 282, (3), H1111-H1117. (doi:10.1152/ajpheart.00391.2001). (PMID:11834510).
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Although platelets have been implicated in the pathogenesis of vascular diseases, little is known about factors that regulate interactions between platelets and the vessel wall under physiological conditions. The objectives of this study were to 1) define the contribution of nitric oxide (NO) to endotoxin (lipopolysaccharide, LPS)-induced platelet-endothelial cell (P/E) adhesion in murine intestinal venules and 2) determine whether the antiadhesive action of NO is mediated by soluble guanylate cyclase (sGC). Adhesive interactions between platelets and endothelial cells were monitored by intravital microscopy. LPS administration into control wild-type mice (WT) resulted in a >15-fold increase in P/E adhesion. Similar responses were observed using endothelial NO synthase (eNOS)-deficient platelets. However, treatment with the NO donor diethylenetriamine-nitric oxide (DETA-NO) attenuated the P/E adhesion response to LPS, whereas the NO synthase inhibitor N(G)-nitro-L-arginine methyl ester or eNOS deficiency resulted in an exacerbation. P/E adhesion response did not differ between LPS-treated WT and inducible NOS-deficient mice. Inhibition of sGC abolished the attenuating effects of DETA-NO, whereas the sGC activator 3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole (YC-1) reduced LPS-induced P/E adhesion. These findings indicate that 1) eNOS-derived NO attenuates endotoxin-induced P/E adhesion and 2) sGC is responsible for the antiadhesive action of NO.
|Keywords:||endotoxemia, nitric oxide synthase, soluble guanylate cyclase, postcapillary venules|
|Subjects:||Q Science > QP Physiology
Q Science > QR Microbiology > QR180 Immunology
|Divisions:||Faculty of Medicine > Infection, Inflammation and Immunity
|Date Deposited:||22 Jun 2012 13:25|
|Last Modified:||22 Jun 2012 13:30|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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