Opposite effects of nitric oxide and nitroxyl on postischemic myocardial injury
Ma, Xin L., Gao, Feng, Liu, Gao-Lin, Lopez, Bernard L., Christopher, Theodore A., Fukuto, Jon M. , Wink, David A. and Feelisch, Martin (1999) Opposite effects of nitric oxide and nitroxyl on postischemic myocardial injury. Proceedings of the National Academy of Sciences of the United States of America, 96, (25), 14617-14622. (doi:10.1073/pnas.96.25.14617). (PMID:10588754).
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Recent experimental evidence suggests that reactive nitrogen oxide species can contribute significantly to postischemic myocardial injury. The aim of the present study was to evaluate the role of two reactive nitrogen oxide species, nitroxyl (NO(-)) and nitric oxide (NO(.)), in myocardial ischemia and reperfusion injury. Rabbits were subjected to 45 min of regional myocardial ischemia followed by 180 min of reperfusion. Vehicle (0.9% NaCl), 1 micromol/kg S-nitrosoglutathione (GSNO) (an NO(.) donor), or 3 micromol/kg Angeli's salt (AS) (a source of NO(-)) were given i.v. 5 min before reperfusion. Treatment with GSNO markedly attenuated reperfusion injury, as evidenced by improved cardiac function, decreased plasma creatine kinase activity, reduced necrotic size, and decreased myocardial myeloperoxidase activity. In contrast, the administration of AS at a hemodynamically equieffective dose not only failed to attenuate but, rather, aggravated reperfusion injury, indicated by an increased left ventricular end diastolic pressure, myocardial creatine kinase release and necrotic size. Decomposed AS was without effect. Co-administration of AS with ferricyanide, a one-electron oxidant that converts NO(-) to NO(.), completely blocked the injurious effects of AS and exerted significant cardioprotective effects similar to those of GSNO. These results demonstrate that, although NO(.) is protective, NO(-) increases the tissue damage that occurs during ischemia/reperfusion and suggest that formation of nitroxyl may contribute to postischemic myocardial injury.
|Digital Object Identifier (DOI):||doi:10.1073/pnas.96.25.14617|
|Subjects:||Q Science > QP Physiology
Q Science > QR Microbiology > QR180 Immunology
|Divisions:||Faculty of Medicine > Clinical and Experimental Sciences
|Date Deposited:||29 Jun 2012 11:01|
|Last Modified:||31 Mar 2016 14:27|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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