Mechanisms of histamine-induced coronary vasodilatation: H1-receptor-mediated release of endothelium-derived nitric oxide

Kelm, Malte, Feelisch, Martin, Krebber, Thomas, Motz, Wolfgang and Strauer, Bodo E. (1993) Mechanisms of histamine-induced coronary vasodilatation: H1-receptor-mediated release of endothelium-derived nitric oxide. Journal of Vascular Research, 30, (3), 132-138. (doi:10.1159/000158987). (PMID:8518330).


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Although the content of histamine in myocardial tissue is high, its contribution to the regulation of coronary blood flow has not been clearly defined. The aim of the present study was to investigate whether or not nitric oxide (NO), an important modulator of coronary vascular tone, is involved in histamine-induced coronary vasomotion and to characterize which histaminergic receptor subtype mediates this process. Isolated, constant-flow-perfused guinea pig hearts were challenged with histamine, the H1-receptor agonist pyridylethylamine (PYR) and the H2-receptor agonist dimaprit (DIM). Apart from coronary perfusion pressure (CPP), left ventricular pressure (LVP) and the development of contractile force (dp/dt), the release of NO and cyclic GMP (cGMP) were continuously measured. Histamine and DIM induced concentration dependently a coronary vasodilatation with an almost 50% decrease in CPP paralleled by an enhancement of LVP and dp/dt by more than 80%. PYR selectively reduced CPP by 47% without affecting LVP and dp/dt. Histamine- and PYR-induced coronary vasodilatation were paralleled by a more-than-twofold increase in basal cGMP release from isolated hearts, whereas DIM exerted no effects on cGMP release. Oxyhemoglobin (4 microM), an effective scavenger of NO, shifted the concentration-response curve for histamine- and PYR-induced changes in CPP significantly to the right and in parallel inhibited the increase in cGMP release. Histamine and PYR rapidly (within 2 s) decreased CPP, while the onset of DIM-induced coronary vasodilatation followed changes in LVP with a lag period of 10 s. Histamine increased basal NO release concentration dependently by a maximum of 351 +/- 21 pmol/min.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1159/000158987
ISSNs: 1018-1172 (print)
1423-0135 (electronic)
Related URLs:
Keywords: histamine, endothelium, nitric oxide, cGMP, coronary circulation
Subjects: R Medicine > R Medicine (General)
Divisions : Faculty of Medicine > Clinical and Experimental Sciences
ePrint ID: 337911
Accepted Date and Publication Date:
Date Deposited: 30 May 2012 09:43
Last Modified: 31 Mar 2016 14:27

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