Quantitative and kinetic characterization of nitric oxide and EDRF released from cultured endothelial cells


Kelm, Malte, Feelisch, Martin, Spahr, Rolf, Piper, Hans-Michael, Noack, Eike and Schrader, Jürgen (1988) Quantitative and kinetic characterization of nitric oxide and EDRF released from cultured endothelial cells. Biochemical and Biophysical Research Communications, 154, (1), 236-244. (doi:10.1016/0006-291X(88)90675-4). (PMID:3260776).

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Description/Abstract

Endothelial cells (EC) contribute to the control of local vascular diameter by formation of an endothelium derived relaxant factor (EDRF) (1). Whether nitric oxide (NO) is identical with (EDRF) or might represent only one species of several EDRFs has not been decided as yet (2-5). Therefore, we have directly compared in cultured EC the kinetics of NO formation determined in a photometric assay with the vasodilatory effect of EDRF and NO in a bioassay. Basal release of NO was 16, 4 pmol/min/ml packed EC column. After stimulation with bradykinin (BK) and ATP onset of endothelial NO release and maximal response preceded the EDRF-mediated relaxation. Concentrations of NO formed by stimulated EC were quantitatively sufficient to fully explain the smooth muscle relaxation determined in the bioassay. Our data provide convincing evidence that under basal, BK and ATP-stimulated conditions 1. endothelial cells release nitric oxide as free radical, 2. nitric oxide is solely responsible for the vasodilatory properties of EDRF.

Item Type: Article
ISSNs: 0006-291X (print)
1090-2104 (electronic)
Related URLs:
Subjects: Q Science > QD Chemistry
R Medicine > R Medicine (General)
R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Medicine > Infection, Inflammation and Immunity
ePrint ID: 337923
Date Deposited: 14 Jun 2012 11:37
Last Modified: 27 Mar 2014 20:21
URI: http://eprints.soton.ac.uk/id/eprint/337923

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