Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles
Kendall, M., Ding, P, Mackay, Rose-Marie, Madsen, Jens, Deb, R., Mackenzie, Z, Kendall, K. and Clark, H.W. (2012) Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles. Nanotoxicology (doi:10.3109/17435390.2012.689880). (PMID:22551051). (In Press).
Full text not available from this repository.
Surfactant protein D (SP-D) is primarily expressed in the lungs and modulates pro- and anti- inflammatory processes to toxic challenge, maintaining lung homeostasis. We investigated the interaction between NPs and SP-D and subsequent uptake by cells involved in lung immunity. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) measuredNP aggregation, particle size and charge in native human SP-D (NhSP-D) and recombinant fragment SP-D (rfhSP-D). Immunohistochemical analysis of A549 epithelial cells investigated the co-localization of NPs and rfhSP-D. NP uptake by alveolar macrophages (AMs) and lung dendritic cells (LDCs) from C57BL/6 and SP-D knock-out mice were compared.SP-D aggregated NPs, especially following the addition of calcium. rfhSP-D enhanced the co-localisation of NPs to epithelial A459 cells in vitro. AMs and LDCs showed decreased uptake of NPs in SP-D deficient mice compared to wild-type mice. These data confirmed an interaction between SP-D and NPs, and subsequent enhanced NP uptake.
|Subjects:||R Medicine > RB Pathology
R Medicine > RC Internal medicine
|Divisions:||Faculty of Medicine > Infection, Inflammation and Immunity
|Date Deposited:||17 May 2012 14:30|
|Last Modified:||17 May 2012 14:32|
|Contributors:||Kendall, M. (Author)
Ding, P (Author)
Mackay, Rose-Marie (Author)
Madsen, Jens (Author)
Deb, R. (Author)
Mackenzie, Z (Author)
Kendall, K. (Author)
Clark, H.W. (Author)
|Date:||2 May 2012|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
Actions (login required)