Cell migration and invasion assays

Moutasim, Karwan A., Nystrom, Maria L. and Thomas, Gareth J. (2011) Cell migration and invasion assays. [in special issue: Cancer Cell Culture] Methods in Molecular Biology, 731, 333-343. (doi:10.1007/978-1-61779-080-5_27). (PMID:21516419).


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A number of in vitro assays have been developed to study tumor cell motility. Historically, assays have been mainly monocellular, where carcinoma cells are studied in isolation. Scratch assays can be used to study the collective and directional movement of populations of cells, whereas two chamber assays lend themselves to the analysis of chemotactic/haptotactic migration and cell invasion. However, an inherent disadvantage of these assays is that they grossly oversimplify the complex process of invasion, lacking the tumor structural architecture and stromal components. Organotypic assays, where tumor cells are grown at an air/liquid interface on gels populated with stromal cells, are a more physiologically relevant method for studying 3-dimensional tumor invasion.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1007/978-1-61779-080-5_27
ISSNs: 1064-3745 (print)
1940-6029 (electronic)
Keywords: invasion, migration, scratch assay, transwell, matrix, organotypic culture
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions : Faculty of Medicine > Cancer Sciences
ePrint ID: 339691
Accepted Date and Publication Date:
Date Deposited: 29 May 2012 11:11
Last Modified: 31 Mar 2016 14:29
URI: http://eprints.soton.ac.uk/id/eprint/339691

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