TiO2-ZrO2 affinity chromatography polymeric microchip for phosphopeptide enrichment and separation

Tsougeni, Katerina, Zerefos, Panagiotis, Tserepi, Angeliki, Vlahou, Antonia, Garbis, Spiros D. and Gogolides, Evangelos (2011) TiO2-ZrO2 affinity chromatography polymeric microchip for phosphopeptide enrichment and separation. Lab on a Chip, 11, (18), 3113-3120. (doi:10.1039/C1LC20133F). (PMID:21796280).


Full text not available from this repository.


We fabricated a TiO(2)-ZrO(2) affinity chromatography micro-column on 2 mm PMMA plates, and demonstrated the enrichment and separation of (a) a standard mono- and tetra-phosphopeptide, and (b) phosphopeptides contained in a tryptic digest of ?-Casein. The chromatography column consisted of 32 parallel microchannels with common input and output ports and was fabricated by lithography directly on the polymeric substrate followed by plasma etching (i.e. standard MEMS processing) and sealed with lamination. The liquid deposited TiO(2)-ZrO(2) stationary phase was characterized by X-ray diffraction and was found to be mostly TiO(2) and ZrO(2) in crystalline phases. Off-chip UV detection and MALDI MS identification of the separated effluents were used. The chip had a capacity of >1.4 ?g (0.7 nmol) of a prototype mono-phosphopeptide and a recovery of 94 ± 3%, and can be used with small samples (less than 0.1 ?L depending on the syringe pump used). The chip design allows an expansion of its capacity by means of increasing the number of parallel microchannels at a constant sample volume. Our approach provided an alternative to off-line extraction tips (with typical capacities of 1-2 ?g and sample volumes of 1-10 ?L), and to on-chip efforts based on packed bed and frit formats.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1039/C1LC20133F
ISSNs: 1473-0197 (print)
1473-0189 (electronic)
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions : Faculty of Medicine > Cancer Sciences
ePrint ID: 339700
Accepted Date and Publication Date:
21 September 2011Published
Date Deposited: 29 May 2012 11:28
Last Modified: 31 Mar 2016 14:29
URI: http://eprints.soton.ac.uk/id/eprint/339700

Actions (login required)

View Item View Item