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Recruitment of Cln3 cyclin to promoters controls cell cycle entry via histone deacetylase and other targets

Recruitment of Cln3 cyclin to promoters controls cell cycle entry via histone deacetylase and other targets
Recruitment of Cln3 cyclin to promoters controls cell cycle entry via histone deacetylase and other targets
In yeast, the G1 cyclin Cln3 promotes cell cycle entry by activating the transcription factor SBF. In mammals, there is a parallel system for cell cycle entry in which cyclin dependent kinase (CDK) activates transcription factor E2F/Dp. Here we show that Cln3 regulates SBF by at least two different pathways, one involving the repressive protein Whi5, and the second involving Stb1. The Rpd3 histone deacetylase complex is also involved. Cln3 binds to SBF at the CLN2 promoter, and removes previously bound Whi5 and histone deacetylase. Adding extra copies of the SBF binding site to the cell delays Start, possibly by titrating Cln3. Since Rpd3 is the yeast ortholog of mammalian HDAC1, there is now a virtually complete analogy between the proteins regulating cell cycle entry in yeast (SBF, Cln3, Whi5 and Stb1, Rpd3) and mammals (E2F, Cyclin D, Rb, HDAC1). The cell may titrate Cln3 molecules against the number of SBF binding sites, and this could be the underlying basis of the size-control mechanism for Start.
1544-9173
e1000189-[14pp]
Wang, Hongyin
779d6e41-e4b6-4788-a07f-35f3ea1bea79
Carey, Lucas B.
6dfe858d-f195-49ed-a1ee-2ef57323de06
Cai, Ying
80d1cebe-74ff-415d-8950-dae551a4857f
Wijnen, Herman
67e9bc5d-de6e-44ec-b4c2-50b67c5bc79d
Futcher, Bruce
68814b04-75e6-4d33-b228-31b1b557122a
Wang, Hongyin
779d6e41-e4b6-4788-a07f-35f3ea1bea79
Carey, Lucas B.
6dfe858d-f195-49ed-a1ee-2ef57323de06
Cai, Ying
80d1cebe-74ff-415d-8950-dae551a4857f
Wijnen, Herman
67e9bc5d-de6e-44ec-b4c2-50b67c5bc79d
Futcher, Bruce
68814b04-75e6-4d33-b228-31b1b557122a

Wang, Hongyin, Carey, Lucas B., Cai, Ying, Wijnen, Herman and Futcher, Bruce (2009) Recruitment of Cln3 cyclin to promoters controls cell cycle entry via histone deacetylase and other targets. PLoS Biology, 7 (9), e1000189-[14pp]. (doi:10.1371/journal.pbio.1000189). (PMID:19823669)

Record type: Article

Abstract

In yeast, the G1 cyclin Cln3 promotes cell cycle entry by activating the transcription factor SBF. In mammals, there is a parallel system for cell cycle entry in which cyclin dependent kinase (CDK) activates transcription factor E2F/Dp. Here we show that Cln3 regulates SBF by at least two different pathways, one involving the repressive protein Whi5, and the second involving Stb1. The Rpd3 histone deacetylase complex is also involved. Cln3 binds to SBF at the CLN2 promoter, and removes previously bound Whi5 and histone deacetylase. Adding extra copies of the SBF binding site to the cell delays Start, possibly by titrating Cln3. Since Rpd3 is the yeast ortholog of mammalian HDAC1, there is now a virtually complete analogy between the proteins regulating cell cycle entry in yeast (SBF, Cln3, Whi5 and Stb1, Rpd3) and mammals (E2F, Cyclin D, Rb, HDAC1). The cell may titrate Cln3 molecules against the number of SBF binding sites, and this could be the underlying basis of the size-control mechanism for Start.

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e-pub ahead of print date: 8 September 2009
Published date: September 2009
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 340369
URI: http://eprints.soton.ac.uk/id/eprint/340369
ISSN: 1544-9173
PURE UUID: 8bbb9115-57fb-455e-8bc0-13dcfe857b4b
ORCID for Herman Wijnen: ORCID iD orcid.org/0000-0002-8710-5176

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Date deposited: 19 Jun 2012 15:53
Last modified: 15 Mar 2024 03:44

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Contributors

Author: Hongyin Wang
Author: Lucas B. Carey
Author: Ying Cai
Author: Herman Wijnen ORCID iD
Author: Bruce Futcher

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