Analysis of matrix metalloproteinase secretion by macrophages
Elkington, Paul T., Green, Justin A. and Friedland, Jon S. (2009) Analysis of matrix metalloproteinase secretion by macrophages. Methods in Molecular Biology, 531, 253-265. (doi:10.1007/978-1-59745-396-7_16). (PMID:19347322).
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Matrix metalloproteinases (MMPs) are zinc-dependent proteases whose physiological roles include control of leukocyte migration. They are implicated in tissue destruction in inflammatory and infectious diseases. MMPs are not only capable of degrading all components of the extracellular matrix, but they also can modulate the immune response by cleaving cytokines and chemokines to alter their activity. Macrophages secrete a broad range of MMPs and represent a key source of MMPs in inflammatory lesions such as granulomas. Zymography is substrate-based gel electrophoresis that allows direct visualization of MMP activity. Here we describe measurement of MMP secretion from macrophages focusing on quantitative zymography. We also discuss complementary methods that should be used in parallel with zymography. The ability to analyze and quantify MMP secretion by macrophages offers an additional window through which to understand the contributions of macrophages to a wide variety of infectious, inflammatory, and immunologic disorders.
|Subjects:||Q Science > QR Microbiology > QR180 Immunology
Q Science > QR Microbiology > QR355 Virology
|Divisions:||Faculty of Medicine
|Date Deposited:||12 Jul 2012 09:30|
|Last Modified:||27 Mar 2014 20:23|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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