Developmental cues for bone formation from parathyroid hormone and parathyroid hormone-related protein in an ex vivo organotypic culture system of embryonic chick femora


Smith, Emma L., Kanczler, Janos M., Roberts, Carol A. and Oreffo, Richard O. C. (2012) Developmental cues for bone formation from parathyroid hormone and parathyroid hormone-related protein in an ex vivo organotypic culture system of embryonic chick femora. Tissue Engineering Part C Methods, 18, (12), 984-994. (doi:10.1089/ten.tec.2012.0132). (PMID:22690868).

Download

Full text not available from this repository.

Description/Abstract

Enhancement and application of our understanding of skeletal developmental biology is critical to developing tissue engineering approaches to bone repair. We propose that use of the developing embryonic femur as a model to further understand skeletogenesis, and the effects of key differentiation agents, will aid our understanding of the developing bone niche and inform bone reparation. We have used a three-dimensional organotypic culture system of embryonic chick femora to investigate the effects of two key skeletal differentiation agents, parathyroid hormone (PTH) and parathyroid hormone-related protein (PTHrP), on bone and cartilage development, using a combination of microcomputed tomography and histological analysis to assess tissue formation and structure, and cellular behavior. Stimulation of embryonic day 11 (E11) organotypic femur cultures with PTH and PTHrP initiated osteogenesis. Bone formation was enhanced, with increased collagen I and STRO-1 expression, and cartilage was reduced, with decreased chondrocyte proliferation, collagen II expression, and glycosaminoglycan levels. This study demonstrates the successful use of organotypic chick femur cultures as a model for bone development, evidenced by the ability of exogenous bioactive molecules to differentially modulate bone and cartilage formation. The organotypic model outlined provides a tool for analyzing key temporal stages of bone and cartilage development, providing a paradigm for translation of bone development to improve scaffolds and skeletal stem cell treatments for skeletal regenerative medicine.

Item Type: Article
ISSNs: 1937-3384 (print)
1937-3392 (electronic)
Subjects: Q Science > QP Physiology
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine > Human Development and Health
ePrint ID: 345790
Date Deposited: 03 Dec 2012 15:55
Last Modified: 28 Mar 2014 15:27
Research Funder: BBSRC
Projects:
Combining stem cell science and tissue engineering to study the development and repair of human skeletal tissue
Funded by: BBSRC (BB/G010579/1)
Led by: Richard Oreffo
1 October 2009 to 30 September 2014
URI: http://eprints.soton.ac.uk/id/eprint/345790

Actions (login required)

View Item View Item