Translational hurdles for tissue engineering: an in vitro analysis of commonly used local anaesthetics on skeletal stem cell survival

Tayton, E.R., Smith, J.O., Aarvold, A., Kalra, S., Dunlop, D.G. and Oreffo, Richard O. C. (2012) Translational hurdles for tissue engineering: an in vitro analysis of commonly used local anaesthetics on skeletal stem cell survival. The Journal of Bone and Joint Surgery, 94, (6), 848-855. (doi:10.1302/0301-620X.94B6.28479). (PMID:22628604).


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When transferring tissue regenerative strategies involving skeletal stem cells to human application, consideration needs to be given to factors that may affect the function of the cells that are transferred. Local anaesthetics are frequently used during surgical procedures, either administered directly into the operative site or infiltrated subcutaneously around the wound. The aim of this study was to investigate the effects of commonly used local anaesthetics on the morphology, function and survival of human adult skeletal stem cells. Cells from three patients who were undergoing elective hip replacement were harvested and incubated for two hours with 1% lidocaine, 0.5% levobupivacaine or 0.5% bupivacaine hydrochloride solutions. Viability was quantified using WST-1 and DNA assays. Viability and morphology were further characterised using CellTracker Green/Ethidium Homodimer-1 immunocytochemistry and function was assessed by an alkaline phosphatase assay. An additional group was cultured for a further seven days to allow potential recovery of the cells after removal of the local anaesthetic. A statistically significant and dose dependent reduction in cell viability and number was observed in the cell cultures exposed to all three local anaesthetics at concentrations of 25% and 50%, and this was maintained even following culture for a further seven days. This study indicates that certain local anaesthetic agents in widespread clinical use are deleterious to skeletal progenitor cells when studied in vitro; this might have relevance in clinical applications.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1302/0301-620X.94B6.28479
ISSNs: 0021-9355 (print)
1535-1386 (electronic)
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QM Human anatomy
R Medicine > RM Therapeutics. Pharmacology
Divisions : Faculty of Medicine > Human Development and Health
ePrint ID: 345792
Accepted Date and Publication Date:
June 2012Published
Date Deposited: 03 Dec 2012 16:30
Last Modified: 31 Mar 2016 14:38
Device for Enrichment of Skeletal Stem Cells for Orthopaedic Applications
Funded by: EPSRC (TS/G001650/1)
Led by: Richard Oreffo
1 March 2009 to 28 February 2011

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