The University of Southampton
University of Southampton Institutional Repository

Skeletal stem cell physiology on functionally distinct titania nanotopographies

Skeletal stem cell physiology on functionally distinct titania nanotopographies
Skeletal stem cell physiology on functionally distinct titania nanotopographies
Functionalisation of the surface of orthopaedic implants with nanotopographies that could stimulate in situ osteogenic differentiation of the patient's stem or osteoprogenitor cells would have significant therapeutic potential. Mesenchymal stem cell (MSC) responses to titanium substrates patterned with nanopillar structures were investigated in this study. Focal adhesions were quantified in S-phase cells, the bone-related transcription factor Runx2 was examined, osteocalcin production was noted, and Haralick computational analysis was used to assess the relatedness of the cell responses to each of the titanium substrata based on cytoskeletal textural features. Metabolomics was used as a novel means of assessing cellular responses to the biomaterial substrates by analysing the global metabolite profile of the cells on the substrata, and shows promise as a technique with high data yield for evaluating cell interactions with materials of different surface chemistry or topography. The cell response to 15 nm high nanopillars was distinct, consistent with a transition from a more quiescent phenotype on the planar substrate, to an 'active' phenotype on the pillars. These studies illustrate the potential for clinically relevant titania nanopillared substrata to modulate MSCs, with implications for orthopaedic device design and application.
0142-9612
7403-7410
McNamara, Laura E.
c5efd583-f6a3-4907-b357-1ccf4dba31cb
Sjöström, Terje
9d6734d1-6159-4b03-8e0f-504830018ddf
Burgess, Karl E.V.
7eef15f0-15b2-4898-ac8a-bb5f49f8ff48
Kim, Joseph J.W.
7ff479bc-83ab-45ba-9859-3901c2e7746f
Liu, Er
f9297b4e-5562-4066-9fa5-c6b158aff499
Gordonov, Simon
13d3eb56-e064-45a2-a74d-24aece1aa1e2
Moghe, Prabhas V.
cc4444e6-91c3-4fef-b6c4-b1ed069a3704
Meek, R.M. Dominic
d6875011-a538-4881-8170-baea6a02fac4
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Su, Bo
37e0f5b2-72ab-48e5-87c0-cabdfd83cc21
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
McNamara, Laura E.
c5efd583-f6a3-4907-b357-1ccf4dba31cb
Sjöström, Terje
9d6734d1-6159-4b03-8e0f-504830018ddf
Burgess, Karl E.V.
7eef15f0-15b2-4898-ac8a-bb5f49f8ff48
Kim, Joseph J.W.
7ff479bc-83ab-45ba-9859-3901c2e7746f
Liu, Er
f9297b4e-5562-4066-9fa5-c6b158aff499
Gordonov, Simon
13d3eb56-e064-45a2-a74d-24aece1aa1e2
Moghe, Prabhas V.
cc4444e6-91c3-4fef-b6c4-b1ed069a3704
Meek, R.M. Dominic
d6875011-a538-4881-8170-baea6a02fac4
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Su, Bo
37e0f5b2-72ab-48e5-87c0-cabdfd83cc21
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc

McNamara, Laura E., Sjöström, Terje, Burgess, Karl E.V., Kim, Joseph J.W., Liu, Er, Gordonov, Simon, Moghe, Prabhas V., Meek, R.M. Dominic, Oreffo, Richard O.C., Su, Bo and Dalby, Matthew J. (2011) Skeletal stem cell physiology on functionally distinct titania nanotopographies. Biomaterials, 32 (30), 7403-7410. (doi:10.1016/j.biomaterials.2011.06.063). (PMID:21820172)

Record type: Article

Abstract

Functionalisation of the surface of orthopaedic implants with nanotopographies that could stimulate in situ osteogenic differentiation of the patient's stem or osteoprogenitor cells would have significant therapeutic potential. Mesenchymal stem cell (MSC) responses to titanium substrates patterned with nanopillar structures were investigated in this study. Focal adhesions were quantified in S-phase cells, the bone-related transcription factor Runx2 was examined, osteocalcin production was noted, and Haralick computational analysis was used to assess the relatedness of the cell responses to each of the titanium substrata based on cytoskeletal textural features. Metabolomics was used as a novel means of assessing cellular responses to the biomaterial substrates by analysing the global metabolite profile of the cells on the substrata, and shows promise as a technique with high data yield for evaluating cell interactions with materials of different surface chemistry or topography. The cell response to 15 nm high nanopillars was distinct, consistent with a transition from a more quiescent phenotype on the planar substrate, to an 'active' phenotype on the pillars. These studies illustrate the potential for clinically relevant titania nanopillared substrata to modulate MSCs, with implications for orthopaedic device design and application.

This record has no associated files available for download.

More information

Published date: October 2011
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 346009
URI: http://eprints.soton.ac.uk/id/eprint/346009
ISSN: 0142-9612
PURE UUID: c7d0b0d9-5e0a-4958-b49b-203d25335a6f
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 11 Dec 2012 11:46
Last modified: 15 Mar 2024 03:04

Export record

Altmetrics

Contributors

Author: Laura E. McNamara
Author: Terje Sjöström
Author: Karl E.V. Burgess
Author: Joseph J.W. Kim
Author: Er Liu
Author: Simon Gordonov
Author: Prabhas V. Moghe
Author: R.M. Dominic Meek
Author: Bo Su
Author: Matthew J. Dalby

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×