The University of Southampton
University of Southampton Institutional Repository

Primer1: Primer design Web service for Tetra-Primer ARMS-PCR

Primer1: Primer design Web service for Tetra-Primer ARMS-PCR
Primer1: Primer design Web service for Tetra-Primer ARMS-PCR
Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given sequence. The Primer1 software was developed originally for use in the context of restriction fragment length polymorphism analysis using gel electrophoresis. However, recent applications have been more diverse, reviewed here, and we present an overview of the Primer1 software for primer design and web-service. We have updated the Primer1 program, and provide more complete details of the implementation. We also provide test data and output. The program is now available on a new, efficient, LAMP web service for users at: http://primer1.soton.ac.uk/primer1.html
1875-0362
55-58
Collins, Andrew
7daa83eb-0b21-43b2-af1a-e38fb36e2a64
Ke, Xiayi
3d3489dc-c021-4669-bf5c-30c8733c1183
Collins, Andrew
7daa83eb-0b21-43b2-af1a-e38fb36e2a64
Ke, Xiayi
3d3489dc-c021-4669-bf5c-30c8733c1183

Collins, Andrew and Ke, Xiayi (2012) Primer1: Primer design Web service for Tetra-Primer ARMS-PCR. The Open Bioinformatics Journal, 6, 55-58. (doi:10.2174/1875036201206010055).

Record type: Article

Abstract

Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given sequence. The Primer1 software was developed originally for use in the context of restriction fragment length polymorphism analysis using gel electrophoresis. However, recent applications have been more diverse, reviewed here, and we present an overview of the Primer1 software for primer design and web-service. We have updated the Primer1 program, and provide more complete details of the implementation. We also provide test data and output. The program is now available on a new, efficient, LAMP web service for users at: http://primer1.soton.ac.uk/primer1.html

Text
55TOBIOIJ.pdf - Version of Record
Download (192kB)

More information

Published date: 30 November 2012
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 346184
URI: http://eprints.soton.ac.uk/id/eprint/346184
ISSN: 1875-0362
PURE UUID: 04ee008c-ed8d-4b1e-ab54-a596d25196d4
ORCID for Andrew Collins: ORCID iD orcid.org/0000-0001-7108-0771

Catalogue record

Date deposited: 14 Jan 2013 14:31
Last modified: 15 Mar 2024 02:43

Export record

Altmetrics

Contributors

Author: Andrew Collins ORCID iD
Author: Xiayi Ke

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×