Plasma stencilling methods for cell patterning
Plasma stencilling methods for cell patterning
In this paper we describe plasma stencilling techniques for patterning 10 mammalian cell lines on hydrophobic and cell repellent poly(dimethylsiloxane) (PDMS), methylated glass and bacterial grade polystyrene surfaces. An air plasma produced with a Tesla generator operating at atmospheric pressure was used with micro-engineered stencils for patterned surface oxidation, selectively transforming the surface to a hydrophilic state to enable cell adhesion and growth. Plasma stencilling obviates the need for directly patterning cell adhesion molecules. Instead, during cell culture, adhesion proteins from the media assemble in a bioactive form on the hydrophilic regions. Critically, the removal of protein patterning prior to cell culture provides the option to also use PDMS-PDMS plasma bonding to incorporate cell patterns within microfluidic systems. Linear patterns were generated using PDMS microchannel stencils, and polyimide stencils with through holes were used for the production of cellular arrays. For the production of smaller cellular arrays, a novel microcapillary-based dielectric barrier discharge system was developed. A numerical method to characterise the cell patterns is also introduced and was used to demonstrate that plasma stencilling is highly effective, with complete patterns confined during long term cell culture (>10 days). In summary, plasma stencilling is simple, rapid, inexpensive, reproducible and a potentially universal cell line patterning capability.
Cell patterning, plasmas, stencil, poly(dimethylsiloxane, dielectric barrier discharge, microfluidics, microfluidic networks, surface modification, polystyrene surface, soft lithography, proteins adhesion, serum shape, poly(dimethylsiloxane) micropatterns
601-609
Frimat, J. P.
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Menne, H.
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Michels, A.
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Kittel, S.
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Kettler, R.
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Borgmann, S.
7fa3dbf7-3454-4151-b737-18ecfb0ce04b
Franzke, J.
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West, J.
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17 May 2009
Frimat, J. P.
13af45f9-86bd-4ae2-9459-7f573eae2342
Menne, H.
d4f73469-c0da-4b7e-9398-2e7900fa7055
Michels, A.
03d025f9-dc0d-4dee-a5c9-e1fdd81a8ff9
Kittel, S.
358bbc44-ef66-409d-9f55-0d106f7fd016
Kettler, R.
e06563a9-37f8-4218-8861-5d798e944c6e
Borgmann, S.
7fa3dbf7-3454-4151-b737-18ecfb0ce04b
Franzke, J.
fd24d4c2-387e-4d2d-981c-dcf045aaf41e
West, J.
a54f2efc-9fcf-458d-90a8-fc11b3142fd4
Frimat, J. P., Menne, H., Michels, A., Kittel, S., Kettler, R., Borgmann, S., Franzke, J. and West, J.
(2009)
Plasma stencilling methods for cell patterning.
Analytical and Bioanalytical Chemistry, 395 (3), .
(doi:10.1007/s00216-009-2824-7).
Abstract
In this paper we describe plasma stencilling techniques for patterning 10 mammalian cell lines on hydrophobic and cell repellent poly(dimethylsiloxane) (PDMS), methylated glass and bacterial grade polystyrene surfaces. An air plasma produced with a Tesla generator operating at atmospheric pressure was used with micro-engineered stencils for patterned surface oxidation, selectively transforming the surface to a hydrophilic state to enable cell adhesion and growth. Plasma stencilling obviates the need for directly patterning cell adhesion molecules. Instead, during cell culture, adhesion proteins from the media assemble in a bioactive form on the hydrophilic regions. Critically, the removal of protein patterning prior to cell culture provides the option to also use PDMS-PDMS plasma bonding to incorporate cell patterns within microfluidic systems. Linear patterns were generated using PDMS microchannel stencils, and polyimide stencils with through holes were used for the production of cellular arrays. For the production of smaller cellular arrays, a novel microcapillary-based dielectric barrier discharge system was developed. A numerical method to characterise the cell patterns is also introduced and was used to demonstrate that plasma stencilling is highly effective, with complete patterns confined during long term cell culture (>10 days). In summary, plasma stencilling is simple, rapid, inexpensive, reproducible and a potentially universal cell line patterning capability.
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More information
Published date: 17 May 2009
Additional Information:
ISI Document Delivery No.: 495SV
Times Cited: 21
Cited Reference Count: 44
Frimat, Jean-Philippe Menne, Heike Michels, Antje Kittel, Silke Kettler, Raffael Borgmann, Sabine Franzke, Joachim West, Jonathan
European Community [NMP4-CT-2004-500039]; Ministerium fur Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen; Bundesministerium fur Bildung und Forschung
The authors are grateful to Uli Marggraf for SU-8 master fabrication, Norman Ahlman for white light interferometry, Maria Becker for SEM studies, Helmut Lindner for assistance with the patterning equations, Axel Mescher for providing the microplasma imaging capability and Peter Jacob for support with contact angle measurements. The cell lines were kindly provided by Cristina Cadenas, Sasidhar Maddula and Melissa Mariani. Financial support from the European Community (CellPROM project, Contract No. NMP4-CT-2004-500039) under the 6<SUP>th</SUP> Framework Programme for Research and Technology is gratefully acknowledged. In addition, funding from the Ministerium fur Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen and from the Bundesministerium fur Bildung und Forschung is also greatly appreciated.
Springer heidelberg
Heidelberg
Keywords:
Cell patterning, plasmas, stencil, poly(dimethylsiloxane, dielectric barrier discharge, microfluidics, microfluidic networks, surface modification, polystyrene surface, soft lithography, proteins adhesion, serum shape, poly(dimethylsiloxane) micropatterns
Organisations:
Cancer Sciences
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Local EPrints ID: 346442
URI: http://eprints.soton.ac.uk/id/eprint/346442
ISSN: 1618-2642
PURE UUID: 27b65d91-3d85-4ac1-99b6-63a155d9a534
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Date deposited: 28 Jan 2013 11:17
Last modified: 14 Mar 2024 12:36
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Author:
J. P. Frimat
Author:
H. Menne
Author:
A. Michels
Author:
S. Kittel
Author:
R. Kettler
Author:
S. Borgmann
Author:
J. Franzke
Author:
J. West
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