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High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes

High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes
High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes
Spatially defined neuronal networks have great potential to be used in a wide spectrum of neurobiology assays. We present an original technique for the precise and reproducible formation of neuronal networks. A PDMS membrane comprising through-holes aligned with interconnecting microchannels was used during oxygen plasma etching to dry mask a protein rejecting poly(ethylene glycol) (PEG) adlayer. Patterns were faithfully replicated to produce an oxidized interconnected array pattern which supported protein adsorption. Differentiated human SH-SY5Y neuron-like cells adhered to the array nodes with the micron-scale interconnecting tracks guiding neurite outgrowth to produce neuronal connections and establish a network. A 2.0 mu m track width was optimal for high-level network formation and node compliance. These spatially standardized neuronal networks were used to analyse the dynamics of acrylamide-induced neurite degeneration and the protective effects of co-treatment with calpeptin or brain derived neurotrophic factor (BDNF).
poly(l-lysine)-g-poly(ethylene glycol) layers, metal-oxide surfaces, neurite outgrowth, hippocampal-neurons, protein adsorption, soft lithography, elastomeric membranes, patterned growth, cell-cultures, sh-sy5y cells
1473-0197
2763-2771
Hardelauf, H.
b36ab7ef-db53-478d-9e03-7dee328f0613
Sisnaiske, J.
153da33a-b334-450e-b7f0-2c6942999ac7
Taghipour-Anvari, A.A.
235c002e-10ed-47e0-ad43-975657b85837
Jacob, P.
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Drabiniok, E.
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Marggraf, U.
5e8919fe-0fa9-40ec-91b5-652ac35488e3
Frimat, J.P.
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Hengstler, J.G.
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Neyer, A.
e224b130-503e-46f0-8471-b4370a62a7a5
van Thriel, C.
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West, J.
f1c2e060-16c3-44c0-af70-242a1c58b968
Hardelauf, H.
b36ab7ef-db53-478d-9e03-7dee328f0613
Sisnaiske, J.
153da33a-b334-450e-b7f0-2c6942999ac7
Taghipour-Anvari, A.A.
235c002e-10ed-47e0-ad43-975657b85837
Jacob, P.
c5a1b7e2-0bfe-4916-94c6-c25f20f2f7bc
Drabiniok, E.
5415f1fd-d267-4dae-8c0c-63b150db6bf2
Marggraf, U.
5e8919fe-0fa9-40ec-91b5-652ac35488e3
Frimat, J.P.
e8bb0e6a-729b-48bf-8a3b-5c30f9878832
Hengstler, J.G.
ae4a2b59-c041-4fca-9a66-d0d6a7053371
Neyer, A.
e224b130-503e-46f0-8471-b4370a62a7a5
van Thriel, C.
4462d39a-5c01-4420-98db-0486836dbd1a
West, J.
f1c2e060-16c3-44c0-af70-242a1c58b968

Hardelauf, H., Sisnaiske, J., Taghipour-Anvari, A.A., Jacob, P., Drabiniok, E., Marggraf, U., Frimat, J.P., Hengstler, J.G., Neyer, A., van Thriel, C. and West, J. (2011) High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes. Lab on a Chip, 11 (16), 2763-2771. (doi:10.1039/C1LC20257J).

Record type: Article

Abstract

Spatially defined neuronal networks have great potential to be used in a wide spectrum of neurobiology assays. We present an original technique for the precise and reproducible formation of neuronal networks. A PDMS membrane comprising through-holes aligned with interconnecting microchannels was used during oxygen plasma etching to dry mask a protein rejecting poly(ethylene glycol) (PEG) adlayer. Patterns were faithfully replicated to produce an oxidized interconnected array pattern which supported protein adsorption. Differentiated human SH-SY5Y neuron-like cells adhered to the array nodes with the micron-scale interconnecting tracks guiding neurite outgrowth to produce neuronal connections and establish a network. A 2.0 mu m track width was optimal for high-level network formation and node compliance. These spatially standardized neuronal networks were used to analyse the dynamics of acrylamide-induced neurite degeneration and the protective effects of co-treatment with calpeptin or brain derived neurotrophic factor (BDNF).

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More information

Published date: 28 June 2011
Additional Information: ISI Document Delivery No.: 798AS Times Cited: 11 Cited Reference Count: 71 Hardelauf, Heike Sisnaiske, Julia Taghipour-Anvari, Amir Ali Jacob, Peter Drabiniok, Evelyn Marggraf, Ulrich Frimat, Jean-Philippe Hengstler, Jan G. Neyer, Andreas van Thriel, Christoph West, Jonathan European Community [FP7/2007-2013, HEALTH-F5-2008-201619]; German Research Foundation [WE3737/3-1]; German Federal Ministry of Education and Research [0101-31P6541]; Ministry of Innovation, Science, Research and Technology of the State of North Rhine-Westphalia; International Leibniz Graduate School "Systems Biology Lab-on-a-Chip'' The authors are grateful to Sarah Waide for cell culture support, Silke Kittle for XPS analysis and Maria Becker for SEM imaging. The research was financially supported by the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement no HEALTH-F5-2008-201619 (ESNATS), the German Research Foundation (DFG grant WE3737/3-1), the German Federal Ministry of Education and Research (BMBF grant 0101-31P6541) and the Ministry of Innovation, Science, Research and Technology of the State of North Rhine-Westphalia. Heike Hardelauf thanks the International Leibniz Graduate School "Systems Biology Lab-on-a-Chip'' for financial support. Royal soc chemistry Cambridge
Keywords: poly(l-lysine)-g-poly(ethylene glycol) layers, metal-oxide surfaces, neurite outgrowth, hippocampal-neurons, protein adsorption, soft lithography, elastomeric membranes, patterned growth, cell-cultures, sh-sy5y cells
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 346448
URI: http://eprints.soton.ac.uk/id/eprint/346448
ISSN: 1473-0197
PURE UUID: ef32c9c7-74ca-4eee-b8c4-54e732b14a2f
ORCID for J. West: ORCID iD orcid.org/0000-0002-5709-6790

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Date deposited: 28 Jan 2013 11:59
Last modified: 15 Mar 2024 03:43

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Contributors

Author: H. Hardelauf
Author: J. Sisnaiske
Author: A.A. Taghipour-Anvari
Author: P. Jacob
Author: E. Drabiniok
Author: U. Marggraf
Author: J.P. Frimat
Author: J.G. Hengstler
Author: A. Neyer
Author: C. van Thriel
Author: J. West ORCID iD

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