Preliminary study of the cellular characteristics of primary bronchial fibroblasts in patients with asthma: expression of alpha-smooth muscle actin, fibronectin containing extra type III domain A, and smoothelin.
Preliminary study of the cellular characteristics of primary bronchial fibroblasts in patients with asthma: expression of alpha-smooth muscle actin, fibronectin containing extra type III domain A, and smoothelin.
BACKGROUND: The relationship between fibroblasts, myofibroblasts, and smooth muscle cells within the airway wall remains poorly understood.
OBJECTIVE: The cellular characteristics of primary bronchial fibroblasts from patients with asthma were investigated by evaluating the expression of 3 proteins: alpha-smooth muscle actin (SMA), fibronectin containing extra type III domain A (EDAcFN), and smoothelin.
METHODS: Expression of SMA, EDAcFN, and smoothelin was evaluated in primary fibroblasts from 3 patients with asthma of varying symptom severity, embryonic fibroblasts, and a healthy control. In addition, primary bronchial fibroblasts from patients with asthma were assessed for SMA at various incubation times (4 hours to 76 hours) and with different extracellular matrices (ECMs). Immunofluorescence was assessed by manually counting cells that stained positively as fine filamentous structures under a fluorescence microscope.
RESULTS: Expression of filamentous SMA tended to increase with the length of incubation. The positive to total cell ratio for filamentous cells did not differ significantly between the various kinds of ECMs onto which cells were plated (P > .05). Primary bronchial fibroblasts from asthma patients produced more prominent expression of EDAcFN than control fibroblasts. Smoothelin was not expressed in any fibroblasts.
CONCLUSIONS: More than 50% of primary bronchial fibroblasts were defined as myofibroblasts. Primary bronchial fibroblasts in patients with asthma had more potential for tissue fibrosis than control fibroblasts. No mature smooth muscle cells were observed in primary bronchial fibroblasts in patients with asthma.
asthma, fibroblast, smooth muscle actin, fibronectins
20-27
Nam, Y.-H.
cbe68df5-9db5-4a82-a307-0a2fa8de8049
Lee, S.K.
2bda7741-8d13-42f7-937a-a865fe2df798
Sammut, D.
6802b440-cca3-4fdf-860a-fd279629a1cc
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Howarth, P.H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
2012
Nam, Y.-H.
cbe68df5-9db5-4a82-a307-0a2fa8de8049
Lee, S.K.
2bda7741-8d13-42f7-937a-a865fe2df798
Sammut, D.
6802b440-cca3-4fdf-860a-fd279629a1cc
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Howarth, P.H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Nam, Y.-H., Lee, S.K., Sammut, D., Davies, D.E. and Howarth, P.H.
(2012)
Preliminary study of the cellular characteristics of primary bronchial fibroblasts in patients with asthma: expression of alpha-smooth muscle actin, fibronectin containing extra type III domain A, and smoothelin.
Journal of Investigational Allergology and Clinical Immunology, 22 (1), .
(PMID:22448450)
Abstract
BACKGROUND: The relationship between fibroblasts, myofibroblasts, and smooth muscle cells within the airway wall remains poorly understood.
OBJECTIVE: The cellular characteristics of primary bronchial fibroblasts from patients with asthma were investigated by evaluating the expression of 3 proteins: alpha-smooth muscle actin (SMA), fibronectin containing extra type III domain A (EDAcFN), and smoothelin.
METHODS: Expression of SMA, EDAcFN, and smoothelin was evaluated in primary fibroblasts from 3 patients with asthma of varying symptom severity, embryonic fibroblasts, and a healthy control. In addition, primary bronchial fibroblasts from patients with asthma were assessed for SMA at various incubation times (4 hours to 76 hours) and with different extracellular matrices (ECMs). Immunofluorescence was assessed by manually counting cells that stained positively as fine filamentous structures under a fluorescence microscope.
RESULTS: Expression of filamentous SMA tended to increase with the length of incubation. The positive to total cell ratio for filamentous cells did not differ significantly between the various kinds of ECMs onto which cells were plated (P > .05). Primary bronchial fibroblasts from asthma patients produced more prominent expression of EDAcFN than control fibroblasts. Smoothelin was not expressed in any fibroblasts.
CONCLUSIONS: More than 50% of primary bronchial fibroblasts were defined as myofibroblasts. Primary bronchial fibroblasts in patients with asthma had more potential for tissue fibrosis than control fibroblasts. No mature smooth muscle cells were observed in primary bronchial fibroblasts in patients with asthma.
This record has no associated files available for download.
More information
Published date: 2012
Keywords:
asthma, fibroblast, smooth muscle actin, fibronectins
Organisations:
Clinical & Experimental Sciences
Identifiers
Local EPrints ID: 346687
URI: http://eprints.soton.ac.uk/id/eprint/346687
ISSN: 1018-9068
PURE UUID: 50aa4805-231f-4d6a-9d29-961716d7359a
Catalogue record
Date deposited: 07 Jan 2013 10:39
Last modified: 07 Jul 2022 01:33
Export record
Contributors
Author:
Y.-H. Nam
Author:
S.K. Lee
Author:
D. Sammut
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics