Analysis of nitric oxide-dependent antimicrobial actions in macrophages and mice
Analysis of nitric oxide-dependent antimicrobial actions in macrophages and mice
Nitric oxide (NO*) is a critical component of mammalian host defense that is produced in macrophages and other cells comprising the innate immune system. Isolated mammalian macrophages have been utilized to measure the kinetics of NO production and to demonstrate NO-related antimicrobial actions. Some microorganisms possess enzymes to detoxify nitrogen oxides, and mutant strains lacking these enzymes can be used to demonstrate the importance of these mechanisms for intracellular bacterial survival. This chapter describes techniques with which to analyze the antimicrobial actions of nitric oxide in murine and human macrophages and in laboratory mice.
521-38
Vazquez-Torres, Andrés
41db13e0-ac66-4822-80e8-c9827e521385
Stevanin, Tania
f52978cd-6a4d-45a3-9867-2422493cb7f7
Jones-Carson, Jessica
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Castor, Margaret
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Read, Robert C.
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Fang, Ferric C.
d7a3f05f-37e0-46ce-ae84-5d3234a29590
September 2008
Vazquez-Torres, Andrés
41db13e0-ac66-4822-80e8-c9827e521385
Stevanin, Tania
f52978cd-6a4d-45a3-9867-2422493cb7f7
Jones-Carson, Jessica
a8531adc-1b66-4497-9a83-e4c4e2229b3b
Castor, Margaret
98b7c81d-3bde-42cf-80fb-e9773c346921
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Fang, Ferric C.
d7a3f05f-37e0-46ce-ae84-5d3234a29590
Vazquez-Torres, Andrés, Stevanin, Tania, Jones-Carson, Jessica, Castor, Margaret, Read, Robert C. and Fang, Ferric C.
(2008)
Analysis of nitric oxide-dependent antimicrobial actions in macrophages and mice.
Methods in Enzymology, 437 (6), .
(doi:10.1086/591283).
(PMID:18433645)
Abstract
Nitric oxide (NO*) is a critical component of mammalian host defense that is produced in macrophages and other cells comprising the innate immune system. Isolated mammalian macrophages have been utilized to measure the kinetics of NO production and to demonstrate NO-related antimicrobial actions. Some microorganisms possess enzymes to detoxify nitrogen oxides, and mutant strains lacking these enzymes can be used to demonstrate the importance of these mechanisms for intracellular bacterial survival. This chapter describes techniques with which to analyze the antimicrobial actions of nitric oxide in murine and human macrophages and in laboratory mice.
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Published date: September 2008
Organisations:
Clinical & Experimental Sciences
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Local EPrints ID: 346822
URI: http://eprints.soton.ac.uk/id/eprint/346822
ISSN: 0076-6879
PURE UUID: 0fffdbc2-49d1-40ad-9fdc-e09729bbffa1
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Date deposited: 09 Jan 2013 14:15
Last modified: 15 Mar 2024 03:42
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Contributors
Author:
Andrés Vazquez-Torres
Author:
Tania Stevanin
Author:
Jessica Jones-Carson
Author:
Margaret Castor
Author:
Ferric C. Fang
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