Denaturation of dsDNA immobilised at a negatively charged gold electrode is not caused by electrostatic repulsion
Denaturation of dsDNA immobilised at a negatively charged gold electrode is not caused by electrostatic repulsion
Double-stranded DNA immobilised through a thiol anchor at a gold electrode surface can be unwound and denatured by applying a negative potential. One proposed mechanism for this electrochemical denaturation is that electrostatic field effects are responsible for the destabilisation of the dsDNA through repulsion of the DNA sugar-phosphate backbone away from the electrode surface. Herein, we demonstrate conclusively that electrochemical melting at gold electrodes cannot be explained solely as a simple repulsion mechanism by showing that immobilised DNA denatures at high ionic strengths, where the DNA base-pairs are situated outside of the electrochemical double-layer (and outside the influence of the electric field), and further, that oligomers comprised of the mimic peptide nucleic acid (PNA) can also be denatured at negative potentials, despite the absence of a negatively charged backbone
1625-1632
Johnson, Robert P.
5dabdb76-784a-4d20-bed7-447f2a42705b
Gale, Nittaya
eead6253-2431-407b-ab6b-92e35d41c3ef
Richardson, James A.
5fb4e7f7-2f7e-4e6e-a045-6d7690626695
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
1 April 2013
Johnson, Robert P.
5dabdb76-784a-4d20-bed7-447f2a42705b
Gale, Nittaya
eead6253-2431-407b-ab6b-92e35d41c3ef
Richardson, James A.
5fb4e7f7-2f7e-4e6e-a045-6d7690626695
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Johnson, Robert P., Gale, Nittaya, Richardson, James A., Brown, Tom and Bartlett, Philip N.
(2013)
Denaturation of dsDNA immobilised at a negatively charged gold electrode is not caused by electrostatic repulsion.
Chemical Science, 4, .
(doi:10.1039/c3sc22147d).
Abstract
Double-stranded DNA immobilised through a thiol anchor at a gold electrode surface can be unwound and denatured by applying a negative potential. One proposed mechanism for this electrochemical denaturation is that electrostatic field effects are responsible for the destabilisation of the dsDNA through repulsion of the DNA sugar-phosphate backbone away from the electrode surface. Herein, we demonstrate conclusively that electrochemical melting at gold electrodes cannot be explained solely as a simple repulsion mechanism by showing that immobilised DNA denatures at high ionic strengths, where the DNA base-pairs are situated outside of the electrochemical double-layer (and outside the influence of the electric field), and further, that oligomers comprised of the mimic peptide nucleic acid (PNA) can also be denatured at negative potentials, despite the absence of a negatively charged backbone
Text
Chem Sci c3sc22147d[1].pdf
- Version of Record
Available under License Other.
More information
e-pub ahead of print date: 22 January 2013
Published date: 1 April 2013
Organisations:
Electrochemistry
Identifiers
Local EPrints ID: 348745
URI: http://eprints.soton.ac.uk/id/eprint/348745
ISSN: 1478-6524
PURE UUID: 11ad8d01-b518-46b2-8226-0f4bfa19760f
Catalogue record
Date deposited: 19 Feb 2013 11:14
Last modified: 15 Mar 2024 02:44
Export record
Altmetrics
Contributors
Author:
Robert P. Johnson
Author:
Nittaya Gale
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
