A new modular approach to nanoassembly: stable and addressable DNA nanoconstructs via orthogonal click chemistries
A new modular approach to nanoassembly: stable and addressable DNA nanoconstructs via orthogonal click chemistries
Thermodynamic instability is a problem when assembling and purifying complex DNA nanostructures formed by hybridization alone. To address this issue, we have used photochemical fixation and orthogonal copper-free, ring-strain-promoted, click chemistry for the synthesis of dimeric, trimeric, and oligomeric modular DNA scaffolds from cyclic, double-stranded, 80-mer DNA nanoconstructs. This particular combination of orthogonal click reactions was more effective for nanoassembly than others explored. The complex nanostructures are stable to heat and denaturation agents and can therefore be purified and characterized. They are addressable in a sequence-specific manner by triplex formation, and they can be reversibly and selectively deconstructed. Nanostructures utilizing this orthogonal, chemical fixation methodology can be used as building blocks for nanomachines and functional DNA nanoarchitectures.
dna nanotechnology, photochemical cross-linking, copper-free click, orthogonal chemical fixation
9221-9228
Gerrard, Simon R.
4ed7b304-42c2-497a-b00d-5ac8a1bedd24
Hardiman, Claire
6d3d618a-3554-4897-adcf-9186f41c37f0
Shelbourne, Montserrat
6b004f69-8f1e-4b4d-a8a3-f7dc3f149639
Nandhakumar, Iris
e9850fe5-1152-4df8-8a26-ed44b5564b04
Nordén, Bengt
4a4bbf2f-5acf-41ae-b3ab-7dd93e551038
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
October 2012
Gerrard, Simon R.
4ed7b304-42c2-497a-b00d-5ac8a1bedd24
Hardiman, Claire
6d3d618a-3554-4897-adcf-9186f41c37f0
Shelbourne, Montserrat
6b004f69-8f1e-4b4d-a8a3-f7dc3f149639
Nandhakumar, Iris
e9850fe5-1152-4df8-8a26-ed44b5564b04
Nordén, Bengt
4a4bbf2f-5acf-41ae-b3ab-7dd93e551038
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Gerrard, Simon R., Hardiman, Claire, Shelbourne, Montserrat, Nandhakumar, Iris, Nordén, Bengt and Brown, Tom
(2012)
A new modular approach to nanoassembly: stable and addressable DNA nanoconstructs via orthogonal click chemistries.
ACS Nano, 6 (10), .
(doi:10.1021/nn3035759).
Abstract
Thermodynamic instability is a problem when assembling and purifying complex DNA nanostructures formed by hybridization alone. To address this issue, we have used photochemical fixation and orthogonal copper-free, ring-strain-promoted, click chemistry for the synthesis of dimeric, trimeric, and oligomeric modular DNA scaffolds from cyclic, double-stranded, 80-mer DNA nanoconstructs. This particular combination of orthogonal click reactions was more effective for nanoassembly than others explored. The complex nanostructures are stable to heat and denaturation agents and can therefore be purified and characterized. They are addressable in a sequence-specific manner by triplex formation, and they can be reversibly and selectively deconstructed. Nanostructures utilizing this orthogonal, chemical fixation methodology can be used as building blocks for nanomachines and functional DNA nanoarchitectures.
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e-pub ahead of print date: 18 September 2012
Published date: October 2012
Keywords:
dna nanotechnology, photochemical cross-linking, copper-free click, orthogonal chemical fixation
Organisations:
Molecular Diagnostics & Thereputics
Identifiers
Local EPrints ID: 348776
URI: http://eprints.soton.ac.uk/id/eprint/348776
ISSN: 1936-0851
PURE UUID: 41f978f2-8304-46cd-b652-6c6a9fd935a9
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Date deposited: 19 Feb 2013 14:42
Last modified: 15 Mar 2024 02:57
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Contributors
Author:
Simon R. Gerrard
Author:
Claire Hardiman
Author:
Montserrat Shelbourne
Author:
Bengt Nordén
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