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The calcium sensor calcineurin B-like 9 modulates abscisic acid sensitivity and biosynthesis in Arabidopsis

The calcium sensor calcineurin B-like 9 modulates abscisic acid sensitivity and biosynthesis in Arabidopsis
The calcium sensor calcineurin B-like 9 modulates abscisic acid sensitivity and biosynthesis in Arabidopsis
Calcium plays a pivotal role in plant responses to several stimuli, including pathogens, abiotic stresses, and hormones. However, the molecular mechanisms underlying calcium functions are poorly understood. It is hypothesized that calcium serves as second messenger and, in many cases, requires intracellular protein sensors to transduce the signal further downstream in the pathways. The calcineurin B-like proteins (CBLs) represent a unique family of calcium sensors in plant cells. Here, we report our analysis of the CBL9 member of this gene family. Expression of CBL9 was inducible by multiple stress signals and abscisic acid (ABA) in young seedlings. When CBL9 gene function was disrupted in Arabidopsis thaliana plants, the responses to ABA were drastically altered. The mutant plants became hypersensitive to ABA in the early developmental stages, including seed germination and post-germination seedling growth. In addition, seed germination in the mutant also showed increased sensitivity to inhibition by osmotic stress conditions produced by high concentrations of salt and mannitol. Further analyses indicated that increased stress sensitivity in the mutant may be a result of both ABA hypersensitivity and increased accumulation of ABA under the stress conditions. The cbl9 mutant plants showed enhanced expression of genes involved in ABA signaling, such as ABA-INSENSITIVE 4 and 5. This study has identified a calcium sensor as a common element in the ABA signaling and stress-induced ABA biosynthesis pathways.
1040-4651
1912-1924
Pandey, Girdhar K.
cecd6b35-fc04-443e-a276-f5b4e6997507
Cheong, Yong Hwa
1d213a41-44ca-451b-8c2a-d33e73080e3d
Kim, Kyung-Nam
8c8aba8b-2038-4a40-91b1-ac9f6504ac3c
Grant, John J.
42bdaa6a-023d-420c-8d36-261f3d092f36
Li, Legong
6ec68e8e-3adf-47ce-a86d-c1793e09c920
Hung, Wendy
9e3146b2-bf1c-4e9a-a7e5-40755cb0a11d
D'Angelo, Cecilia
0d35b03b-684d-43aa-a57a-87212ab07ee1
Weinl, Stefan
2f78f0c7-3be9-4b12-8ddf-364731ab5071
Kudla, Jorg
4e8925ae-1d02-4756-b9e9-6844cc074a85
Luan, Sheng
baa8b704-cf99-4c11-8eac-f754b4b83223
Pandey, Girdhar K.
cecd6b35-fc04-443e-a276-f5b4e6997507
Cheong, Yong Hwa
1d213a41-44ca-451b-8c2a-d33e73080e3d
Kim, Kyung-Nam
8c8aba8b-2038-4a40-91b1-ac9f6504ac3c
Grant, John J.
42bdaa6a-023d-420c-8d36-261f3d092f36
Li, Legong
6ec68e8e-3adf-47ce-a86d-c1793e09c920
Hung, Wendy
9e3146b2-bf1c-4e9a-a7e5-40755cb0a11d
D'Angelo, Cecilia
0d35b03b-684d-43aa-a57a-87212ab07ee1
Weinl, Stefan
2f78f0c7-3be9-4b12-8ddf-364731ab5071
Kudla, Jorg
4e8925ae-1d02-4756-b9e9-6844cc074a85
Luan, Sheng
baa8b704-cf99-4c11-8eac-f754b4b83223

Pandey, Girdhar K., Cheong, Yong Hwa, Kim, Kyung-Nam, Grant, John J., Li, Legong, Hung, Wendy, D'Angelo, Cecilia, Weinl, Stefan, Kudla, Jorg and Luan, Sheng (2004) The calcium sensor calcineurin B-like 9 modulates abscisic acid sensitivity and biosynthesis in Arabidopsis. The Plant Cell, 16 (7), 1912-1924. (doi:10.1105/tpc.021311). (PMID:15208400)

Record type: Article

Abstract

Calcium plays a pivotal role in plant responses to several stimuli, including pathogens, abiotic stresses, and hormones. However, the molecular mechanisms underlying calcium functions are poorly understood. It is hypothesized that calcium serves as second messenger and, in many cases, requires intracellular protein sensors to transduce the signal further downstream in the pathways. The calcineurin B-like proteins (CBLs) represent a unique family of calcium sensors in plant cells. Here, we report our analysis of the CBL9 member of this gene family. Expression of CBL9 was inducible by multiple stress signals and abscisic acid (ABA) in young seedlings. When CBL9 gene function was disrupted in Arabidopsis thaliana plants, the responses to ABA were drastically altered. The mutant plants became hypersensitive to ABA in the early developmental stages, including seed germination and post-germination seedling growth. In addition, seed germination in the mutant also showed increased sensitivity to inhibition by osmotic stress conditions produced by high concentrations of salt and mannitol. Further analyses indicated that increased stress sensitivity in the mutant may be a result of both ABA hypersensitivity and increased accumulation of ABA under the stress conditions. The cbl9 mutant plants showed enhanced expression of genes involved in ABA signaling, such as ABA-INSENSITIVE 4 and 5. This study has identified a calcium sensor as a common element in the ABA signaling and stress-induced ABA biosynthesis pathways.

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Published date: July 2004
Organisations: Ocean and Earth Science

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Local EPrints ID: 349902
URI: http://eprints.soton.ac.uk/id/eprint/349902
ISSN: 1040-4651
PURE UUID: f99f761c-f665-44be-bea1-22c416e7ed8f

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Date deposited: 13 Mar 2013 12:13
Last modified: 14 Mar 2024 13:18

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Contributors

Author: Girdhar K. Pandey
Author: Yong Hwa Cheong
Author: Kyung-Nam Kim
Author: John J. Grant
Author: Legong Li
Author: Wendy Hung
Author: Stefan Weinl
Author: Jorg Kudla
Author: Sheng Luan

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