Gold nanoparticles-coated SU-8 for sensitive fluorescence-based detections of DNA
Gold nanoparticles-coated SU-8 for sensitive fluorescence-based detections of DNA
SU-8 epoxy-based negative photoresist has been extensively employed as a structural material for fabrication of numerous biological microelectro-mechanical systems (Bio-MEMS) or lab-on-a-chip (LOC) devices. However, SU-8 has a high autofluorescence level that limits sensitivity of microdevices that use fluorescence as the predominant detection workhorse. Here, we show that deposition of a thin gold nanoparticles layer onto the SU-8 surface significantly reduces the autofluorescence of the coated SU-8 surface by as much as 81% compared to bare SU-8. Furthermore, DNA probes can easily be immobilized on the Au surface with high thermal stability. These improvements enabled sensitive DNA detection by simple DNA hybridization down to 1 nM (a two orders of magnitude improvement) or by solid-phase PCR with sub-picomolar sensitivity. The approach is simple and easy to perform, making it suitable for various Bio-MEMs and LOC devices that use SU-8 as a structural material
gold nanoparticles, SU-8, autofluorescence, solid-phase PCR amplification, DNA hybridization
72-82
Cao, C.
867be05c-5e34-48f7-be6e-e5ffbe812573
Birtwell, S.W.
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Hogberg, J.
55006b9f-f728-4172-8227-9594c579ec5c
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Wolff, A.
5f8a17b8-94e7-46a4-9dfa-9f4644e3f78b
Bang, D.D.
ab2140e2-91ec-4bfe-9cb2-cdcfeafd345b
29 November 2012
Cao, C.
867be05c-5e34-48f7-be6e-e5ffbe812573
Birtwell, S.W.
8dca4d3a-2c9e-415c-b9c2-6b3a07bbf5c4
Hogberg, J.
55006b9f-f728-4172-8227-9594c579ec5c
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Wolff, A.
5f8a17b8-94e7-46a4-9dfa-9f4644e3f78b
Bang, D.D.
ab2140e2-91ec-4bfe-9cb2-cdcfeafd345b
Cao, C., Birtwell, S.W., Hogberg, J., Morgan, H., Wolff, A. and Bang, D.D.
(2012)
Gold nanoparticles-coated SU-8 for sensitive fluorescence-based detections of DNA.
[in special issue: Microfluidics Lab on a Chip Platforms for High Performance Diagnostics]
Diagnostics, 2 (4), Winter Issue, .
(doi:10.3390/diagnostics2040072).
Abstract
SU-8 epoxy-based negative photoresist has been extensively employed as a structural material for fabrication of numerous biological microelectro-mechanical systems (Bio-MEMS) or lab-on-a-chip (LOC) devices. However, SU-8 has a high autofluorescence level that limits sensitivity of microdevices that use fluorescence as the predominant detection workhorse. Here, we show that deposition of a thin gold nanoparticles layer onto the SU-8 surface significantly reduces the autofluorescence of the coated SU-8 surface by as much as 81% compared to bare SU-8. Furthermore, DNA probes can easily be immobilized on the Au surface with high thermal stability. These improvements enabled sensitive DNA detection by simple DNA hybridization down to 1 nM (a two orders of magnitude improvement) or by solid-phase PCR with sub-picomolar sensitivity. The approach is simple and easy to perform, making it suitable for various Bio-MEMs and LOC devices that use SU-8 as a structural material
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Published date: 29 November 2012
Keywords:
gold nanoparticles, SU-8, autofluorescence, solid-phase PCR amplification, DNA hybridization
Organisations:
Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 352081
URI: http://eprints.soton.ac.uk/id/eprint/352081
PURE UUID: e3357521-01c2-4c9a-b019-444007d2e175
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Date deposited: 01 May 2013 10:38
Last modified: 15 Mar 2024 03:18
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Contributors
Author:
C. Cao
Author:
S.W. Birtwell
Author:
J. Hogberg
Author:
H. Morgan
Author:
A. Wolff
Author:
D.D. Bang
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