The University of Southampton
University of Southampton Institutional Repository

Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis.

Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis.
Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis.
Two overlapping genomic fragments have been cloned from Chlamydia trachomatis serovar L1 DNA. Sequence determination of 2530 bp has revealed two open reading frames coding for 'cysteine-rich' (Cr) proteins. One of these proteins was confirmed, by analysis of the inferred amino acid sequence, as the 60-kDa Cr outer membrane protein associated with differentiation of reticulate bodies (RBs) into elementary bodies (EBs). The other smaller 15-kDa protein contained a high percentage of methionine and cysteine and may correspond to a reported smaller and co-ordinately synthesised Cr outer-membrane protein also associated with RB to EB differentiation. Sequencing showed three potential stem-loop structures within the 5', 3' and intergenic regions of the cloned fragment. Southern-blot analysis revealed that the cloned fragment is conserved in ten serovars of C. trachomatis and that a strongly cross-hybridising fragment is also present in Chlamydia psittaci.
0378-1119
307-314
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Ward, M.E.
80edb68e-7ce4-4724-9737-f078f8ffbb70
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Ward, M.E.
80edb68e-7ce4-4724-9737-f078f8ffbb70
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77

Clarke, I.N., Ward, M.E. and Lambden, P.R. (1988) Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis. Gene, 71 (2), 307-314. (PMID:3066701)

Record type: Article

Abstract

Two overlapping genomic fragments have been cloned from Chlamydia trachomatis serovar L1 DNA. Sequence determination of 2530 bp has revealed two open reading frames coding for 'cysteine-rich' (Cr) proteins. One of these proteins was confirmed, by analysis of the inferred amino acid sequence, as the 60-kDa Cr outer membrane protein associated with differentiation of reticulate bodies (RBs) into elementary bodies (EBs). The other smaller 15-kDa protein contained a high percentage of methionine and cysteine and may correspond to a reported smaller and co-ordinately synthesised Cr outer-membrane protein also associated with RB to EB differentiation. Sequencing showed three potential stem-loop structures within the 5', 3' and intergenic regions of the cloned fragment. Southern-blot analysis revealed that the cloned fragment is conserved in ten serovars of C. trachomatis and that a strongly cross-hybridising fragment is also present in Chlamydia psittaci.

This record has no associated files available for download.

More information

Published date: 30 November 1988
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352622
URI: http://eprints.soton.ac.uk/id/eprint/352622
ISSN: 0378-1119
PURE UUID: d89f681c-b7e8-4d4c-977c-c626bcfa3570
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

Catalogue record

Date deposited: 03 Jun 2013 14:29
Last modified: 11 Dec 2021 02:35

Export record

Contributors

Author: I.N. Clarke ORCID iD
Author: M.E. Ward
Author: P.R. Lambden

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×