Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene
Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene
The 60-kDa cysteine-rich outer membrane protein genes of Chlamydia psittaci, Chlamydia pneumoniae, and Chlamydia trachomatis have very different 5' ends, but two areas flanking this variable region show absolute sequence conservation. This observation permitted differentiation of the three species of Chlamydia by the polymerase chain reaction (PCR), forming the basis of a diagnostic test for chlamydial infections. The PCR product containing the variable region of the respective 60-kDa CrP genes was also subjected to restriction endonuclease digestion, enabling differentiation of individual type strains of C. psittaci. Differentiation was possible between lymphogranuloma venereum and trachoma isolates of C. trachomatis. The PCR-based diagnostic test was successful with all strains of chlamydiae studied. The PCR primers showed high specificity and did not produce any product with common bacterial pathogens that may share the same sites of infection.
1188-1193
Watson, M.W.
51934130-1422-4ca9-8a54-a0903f943519
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
June 1991
Watson, M.W.
51934130-1422-4ca9-8a54-a0903f943519
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Watson, M.W., Lambden, P.R. and Clarke, I.N.
(1991)
Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene.
Journal of Clinical Microbiology, 29 (6), .
(PMID:1864938)
Abstract
The 60-kDa cysteine-rich outer membrane protein genes of Chlamydia psittaci, Chlamydia pneumoniae, and Chlamydia trachomatis have very different 5' ends, but two areas flanking this variable region show absolute sequence conservation. This observation permitted differentiation of the three species of Chlamydia by the polymerase chain reaction (PCR), forming the basis of a diagnostic test for chlamydial infections. The PCR product containing the variable region of the respective 60-kDa CrP genes was also subjected to restriction endonuclease digestion, enabling differentiation of individual type strains of C. psittaci. Differentiation was possible between lymphogranuloma venereum and trachoma isolates of C. trachomatis. The PCR-based diagnostic test was successful with all strains of chlamydiae studied. The PCR primers showed high specificity and did not produce any product with common bacterial pathogens that may share the same sites of infection.
This record has no associated files available for download.
More information
Published date: June 1991
Organisations:
Faculty of Medicine
Identifiers
Local EPrints ID: 352639
URI: http://eprints.soton.ac.uk/id/eprint/352639
ISSN: 0095-1137
PURE UUID: e8ae4d53-e041-45bd-9e7c-b6fd2d264481
Catalogue record
Date deposited: 04 Jun 2013 11:29
Last modified: 11 Dec 2021 02:35
Export record
Contributors
Author:
M.W. Watson
Author:
P.R. Lambden
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics