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Molecular characterization of the outer capsid spike protein (VP4) gene from human group C rotavirus

Molecular characterization of the outer capsid spike protein (VP4) gene from human group C rotavirus
Molecular characterization of the outer capsid spike protein (VP4) gene from human group C rotavirus
A cDNA copy of the third genomic RNA segment of a noncultivatable human group C rotavirus (Bristol) was generated by single primer amplification. Human group C rotavirus genome segment 3 contains 2283 bp and encodes the VP4 gene with an open reading frame of 2232 nucleotides (744 amino acids) starting at nucleotide 21 and terminating at nucleotide 2251. PCR primers designed from the 5' and 3' terminal sequences of the C/Bristol VP4 gene were used to amplify the corresponding VP4 gene of a human group C rotavirus from Belém, Brazil. Nucleotide sequence comparisons of the Bristol and Belém VP4 genes revealed 45 differences of which only 6 were predicted to give amino acid changes. Alignment of the two human VP4 sequences with the prototype porcine group C/Cowden rotavirus VP4 showed only 71.2% nucleotide sequence identity. Protein sequence alignments showed that the human group C rotavirus VP4 sequences were 8 amino acids longer than the porcine VP4 sequence with an insertion of 6 amino acids, 252NSKLGD257 adjacent to the proposed proteolytic cleavage region (amino acids 231-250). The large overall number of differences between the human and porcine VP4 sequences strongly suggested that the porcine C/Cowden isolate may belong to a different group C rotavirus P "serotype." In contrast the very close similarity of the VP4 sequences of the UK and Brazilian group C rotaviruses support the hypothesis that these human isolates originate from a recent common ancestor.
0042-6822
442-446
Fielding, P.A.
57d398bf-9838-4acf-add5-d077b6bddb46
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Caul, E.O.
3ab60e6e-ca41-4196-a205-4c0075fd3767
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Fielding, P.A.
57d398bf-9838-4acf-add5-d077b6bddb46
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Caul, E.O.
3ab60e6e-ca41-4196-a205-4c0075fd3767
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Fielding, P.A., Lambden, P.R., Caul, E.O. and Clarke, I.N. (1994) Molecular characterization of the outer capsid spike protein (VP4) gene from human group C rotavirus. Virology, 204 (1), 442-446. (doi:10.1006/viro.1994.1551). (PMID:8091676)

Record type: Article

Abstract

A cDNA copy of the third genomic RNA segment of a noncultivatable human group C rotavirus (Bristol) was generated by single primer amplification. Human group C rotavirus genome segment 3 contains 2283 bp and encodes the VP4 gene with an open reading frame of 2232 nucleotides (744 amino acids) starting at nucleotide 21 and terminating at nucleotide 2251. PCR primers designed from the 5' and 3' terminal sequences of the C/Bristol VP4 gene were used to amplify the corresponding VP4 gene of a human group C rotavirus from Belém, Brazil. Nucleotide sequence comparisons of the Bristol and Belém VP4 genes revealed 45 differences of which only 6 were predicted to give amino acid changes. Alignment of the two human VP4 sequences with the prototype porcine group C/Cowden rotavirus VP4 showed only 71.2% nucleotide sequence identity. Protein sequence alignments showed that the human group C rotavirus VP4 sequences were 8 amino acids longer than the porcine VP4 sequence with an insertion of 6 amino acids, 252NSKLGD257 adjacent to the proposed proteolytic cleavage region (amino acids 231-250). The large overall number of differences between the human and porcine VP4 sequences strongly suggested that the porcine C/Cowden isolate may belong to a different group C rotavirus P "serotype." In contrast the very close similarity of the VP4 sequences of the UK and Brazilian group C rotaviruses support the hypothesis that these human isolates originate from a recent common ancestor.

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More information

Published date: October 1994
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352646
URI: http://eprints.soton.ac.uk/id/eprint/352646
ISSN: 0042-6822
PURE UUID: 5ed146fe-ec23-44fa-9b53-e78fa77c926c
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 04 Jun 2013 13:06
Last modified: 15 Mar 2024 02:33

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Contributors

Author: P.A. Fielding
Author: P.R. Lambden
Author: E.O. Caul
Author: I.N. Clarke ORCID iD

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