Phylogenetic evidence for adaptive evolution of dengue viruses in nature
Phylogenetic evidence for adaptive evolution of dengue viruses in nature
A maximum-likelihood approach was used to analyse selection pressures acting on genes from all four serotypes of dengue virus (DEN). A number of amino acid positions were identified within the envelope (E) glycoprotein that have been subject to relatively weak positive selection in both DEN-3 and DEN-4, as well as in two of the five genotypes of DEN-2. No positive selection was detected in DEN-1. In accordance with the function of the E protein as the major antigenic determinant of DEN, the majority of these sites were located in, or near to, potential T- or B-cell epitopes. A smaller number of selected sites was located in other well-defined functional domains of the E protein, suggesting that cell tropism and virus-mediated membrane fusion may also confer fitness advantages to DEN in nature. Several positively selected amino acid substitutions were also identified in the NS2B and NS5 genes of DEN-2, although the cause of this selection is unclear, whereas the capsid, membrane and non-structural genes NS1, NS2A, NS3 and NS4 were all subject to strong functional constraints. Hence, evidence was found for localized adaptive evolution in natural isolates of DEN, revealing that selection pressures differ among serotypes, genotypes and viral proteins.
1679-1689
Twiddy, S.S.
915e5c44-0b82-4c14-8035-66e6b7fe65d7
Woelk, Christopher H.
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Holmes, E.C.
3679de5a-adee-49d6-9605-62c5016c8c26
July 2002
Twiddy, S.S.
915e5c44-0b82-4c14-8035-66e6b7fe65d7
Woelk, Christopher H.
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Holmes, E.C.
3679de5a-adee-49d6-9605-62c5016c8c26
Twiddy, S.S., Woelk, Christopher H. and Holmes, E.C.
(2002)
Phylogenetic evidence for adaptive evolution of dengue viruses in nature.
Journal of General Virology, 83 (7), .
Abstract
A maximum-likelihood approach was used to analyse selection pressures acting on genes from all four serotypes of dengue virus (DEN). A number of amino acid positions were identified within the envelope (E) glycoprotein that have been subject to relatively weak positive selection in both DEN-3 and DEN-4, as well as in two of the five genotypes of DEN-2. No positive selection was detected in DEN-1. In accordance with the function of the E protein as the major antigenic determinant of DEN, the majority of these sites were located in, or near to, potential T- or B-cell epitopes. A smaller number of selected sites was located in other well-defined functional domains of the E protein, suggesting that cell tropism and virus-mediated membrane fusion may also confer fitness advantages to DEN in nature. Several positively selected amino acid substitutions were also identified in the NS2B and NS5 genes of DEN-2, although the cause of this selection is unclear, whereas the capsid, membrane and non-structural genes NS1, NS2A, NS3 and NS4 were all subject to strong functional constraints. Hence, evidence was found for localized adaptive evolution in natural isolates of DEN, revealing that selection pressures differ among serotypes, genotypes and viral proteins.
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Published date: July 2002
Organisations:
Clinical & Experimental Sciences
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Local EPrints ID: 352757
URI: http://eprints.soton.ac.uk/id/eprint/352757
ISSN: 0022-1317
PURE UUID: 4127307f-d32f-4073-a9ac-f13275fa977a
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Date deposited: 04 Jun 2013 14:04
Last modified: 22 Jul 2022 18:32
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Author:
S.S. Twiddy
Author:
Christopher H. Woelk
Author:
E.C. Holmes
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