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Chlamydia psittaci ewe abortion agent: complete nucleotide sequence of the major outer membrane protein gene

Chlamydia psittaci ewe abortion agent: complete nucleotide sequence of the major outer membrane protein gene
Chlamydia psittaci ewe abortion agent: complete nucleotide sequence of the major outer membrane protein gene
The complete nucleotide sequence encoding the major outer membrane protein (MOMP) of Chlamydia psittaci strain A22/M, responsible for enzootic abortion of ewes (EAE), has been determined. An 800bp EcoRI/XbaI fragment containing a portion of the MOMP coding sequence from C. trachomatis serovar L1 was used to probe a ?L47.1 genomic library constructed from DNA obtained from C. psittaci EAE A22/M. The recombinant L47.1/EA1 was selected and contained the entire C. psittaci MOMP gene within a 7.5 kb BamHI fragment. The DNA sequence revealed an open reading frame encoding 402 amino acids, including a 22 amino acid signal peptide, which exhibited 17/22 conservation with the signal peptide of C. trachomatis MOMP. The calculated molecular mass of the C. psittaci MOMP was 43 kDa. A comparison of the MOMP genes of C. psittaci and C. trachomatis revealed only 34% nucleotide sequence homology, but 65% amino acid homology.
0378-1097
229-234
Pickett, M.A.
4b358cb6-2f2e-4691-a314-0e80df48eadb
Everson, J.S.
8f5e2cbc-b8f9-4ba6-9140-d726764d6c14
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Pickett, M.A.
4b358cb6-2f2e-4691-a314-0e80df48eadb
Everson, J.S.
8f5e2cbc-b8f9-4ba6-9140-d726764d6c14
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Pickett, M.A., Everson, J.S. and Clarke, I.N. (1988) Chlamydia psittaci ewe abortion agent: complete nucleotide sequence of the major outer membrane protein gene. FEMS Microbiology Letters, 55 (2), 229-234. (doi:10.1111/j.1574-6968.1988.tb13939.x).

Record type: Article

Abstract

The complete nucleotide sequence encoding the major outer membrane protein (MOMP) of Chlamydia psittaci strain A22/M, responsible for enzootic abortion of ewes (EAE), has been determined. An 800bp EcoRI/XbaI fragment containing a portion of the MOMP coding sequence from C. trachomatis serovar L1 was used to probe a ?L47.1 genomic library constructed from DNA obtained from C. psittaci EAE A22/M. The recombinant L47.1/EA1 was selected and contained the entire C. psittaci MOMP gene within a 7.5 kb BamHI fragment. The DNA sequence revealed an open reading frame encoding 402 amino acids, including a 22 amino acid signal peptide, which exhibited 17/22 conservation with the signal peptide of C. trachomatis MOMP. The calculated molecular mass of the C. psittaci MOMP was 43 kDa. A comparison of the MOMP genes of C. psittaci and C. trachomatis revealed only 34% nucleotide sequence homology, but 65% amino acid homology.

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Published date: 1 October 1988
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 353699
URI: http://eprints.soton.ac.uk/id/eprint/353699
ISSN: 0378-1097
PURE UUID: 0e5b7dc7-df5e-4f93-b4db-80efe5d2513e
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 14 Jun 2013 09:34
Last modified: 15 Mar 2024 02:33

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Contributors

Author: M.A. Pickett
Author: J.S. Everson
Author: I.N. Clarke ORCID iD

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