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Multiplexed suspension array platform for high-throughput protein assays

Multiplexed suspension array platform for high-throughput protein assays
Multiplexed suspension array platform for high-throughput protein assays
A multiplexed suspension array platform, based on SU8 disks patterned with machine-readable binary identification codes is presented. Multiple probe molecules, each attached to individual disks with different unique codes, provide multiplexed detection of targets in a small sample volume. The experimental system consists of a microfluidic chamber for arraying the particles in a manner suitable for high throughput imaging using a simple fluorescent microscope, together with custom software for automated code readout and analysis of assay response. The platform is demonstrated with a multiplexed antibody assay targeting 3 different human inflammatory cytokines. The suitability of the platform for other bio-analytical applications is discussed.
1387-2176
651-657
Birtwell, S.W.
8dca4d3a-2c9e-415c-b9c2-6b3a07bbf5c4
Broder, G.R.
cde7d221-66af-450a-ae06-dc0f67d9e364
Roach, P.L.
ca94060c-4443-482b-af3e-979243488ba9
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Birtwell, S.W.
8dca4d3a-2c9e-415c-b9c2-6b3a07bbf5c4
Broder, G.R.
cde7d221-66af-450a-ae06-dc0f67d9e364
Roach, P.L.
ca94060c-4443-482b-af3e-979243488ba9
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Birtwell, S.W., Broder, G.R., Roach, P.L. and Morgan, H. (2012) Multiplexed suspension array platform for high-throughput protein assays. Biomedical Microdevices, 14 (4), 651-657. (doi:10.1007/s10544-012-9641-z).

Record type: Article

Abstract

A multiplexed suspension array platform, based on SU8 disks patterned with machine-readable binary identification codes is presented. Multiple probe molecules, each attached to individual disks with different unique codes, provide multiplexed detection of targets in a small sample volume. The experimental system consists of a microfluidic chamber for arraying the particles in a manner suitable for high throughput imaging using a simple fluorescent microscope, together with custom software for automated code readout and analysis of assay response. The platform is demonstrated with a multiplexed antibody assay targeting 3 different human inflammatory cytokines. The suitability of the platform for other bio-analytical applications is discussed.

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More information

Published date: August 2012
Organisations: Electronics & Computer Science
Learn more about Electronics & Computer Science research

Identifiers

Local EPrints ID: 354605
URI: http://eprints.soton.ac.uk/id/eprint/354605
DOI: doi:10.1007/s10544-012-9641-z
ISSN: 1387-2176
PURE UUID: e69d3e20-dbac-4ade-b0ac-b86a9d4befb2
ORCID for P.L. Roach: ORCID iD orcid.org/0000-0001-9880-2877
ORCID for H. Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 16 Jul 2013 10:30
Last modified: 15 Mar 2024 03:18

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Contributors

Author: S.W. Birtwell
Author: G.R. Broder
Author: P.L. Roach ORCID iD
Author: H. Morgan ORCID iD

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