Droplets as reaction compartments for protein nanotechnology
Droplets as reaction compartments for protein nanotechnology
Extreme miniaturization of biological and chemical reactions in pico- to nanoliter microdroplets is emerging as an experimental paradigm that enables more experiments to be carried out with much lower sample consumption, paving the way for high-throughput experiments. This review provides the protein scientist with an experimental framework for (a) formation of polydisperse droplets by emulsification or, alternatively, of monodisperse droplets using microfluidic devices; (b) construction of experimental rigs and microfluidic chips for this purpose; and (c) handling and analysis of droplets.
in vitro compartmentalization, directed evolution, protein engineering, microfluidics, water-in-oil emulsion
978-1-62703-353-4
269-286
Devenish, Sean R.A.
c8e86e13-af5e-4654-86ac-f04237dbff47
Kaltenbach, Miriam
a86f3027-d8fa-4f1a-8ee4-7258a3d64b35
Fischlechner, Martin
b3930129-0775-4c05-81c7-475934df97ee
Hollfelder, Florian
a9f01280-f05d-4057-b5d7-85eac249e477
2013
Devenish, Sean R.A.
c8e86e13-af5e-4654-86ac-f04237dbff47
Kaltenbach, Miriam
a86f3027-d8fa-4f1a-8ee4-7258a3d64b35
Fischlechner, Martin
b3930129-0775-4c05-81c7-475934df97ee
Hollfelder, Florian
a9f01280-f05d-4057-b5d7-85eac249e477
Devenish, Sean R.A., Kaltenbach, Miriam, Fischlechner, Martin and Hollfelder, Florian
(2013)
Droplets as reaction compartments for protein nanotechnology.
In,
Gerrard, Juliet A.
(ed.)
Protein Nanotechnology 2nd edition.
(Methods in Molecular Biology, 996)
Totowa, US.
Springer, .
(doi:10.1007/978-1-62703-354-1_16).
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Book Section
Abstract
Extreme miniaturization of biological and chemical reactions in pico- to nanoliter microdroplets is emerging as an experimental paradigm that enables more experiments to be carried out with much lower sample consumption, paving the way for high-throughput experiments. This review provides the protein scientist with an experimental framework for (a) formation of polydisperse droplets by emulsification or, alternatively, of monodisperse droplets using microfluidic devices; (b) construction of experimental rigs and microfluidic chips for this purpose; and (c) handling and analysis of droplets.
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Published date: 2013
Keywords:
in vitro compartmentalization, directed evolution, protein engineering, microfluidics, water-in-oil emulsion
Organisations:
Chemistry
Identifiers
Local EPrints ID: 356332
URI: http://eprints.soton.ac.uk/id/eprint/356332
ISBN: 978-1-62703-353-4
PURE UUID: 8b0fd558-27c9-4919-a6b8-bc8ccb9834a5
Catalogue record
Date deposited: 24 Sep 2013 12:53
Last modified: 14 Mar 2024 14:47
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Contributors
Author:
Sean R.A. Devenish
Author:
Miriam Kaltenbach
Author:
Martin Fischlechner
Author:
Florian Hollfelder
Editor:
Juliet A. Gerrard
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