Overlapping morphologic and immunophenotypic profiles in small B-cell lymphoma. A report of two cases
Overlapping morphologic and immunophenotypic profiles in small B-cell lymphoma. A report of two cases
We present two cases of small B-cell lymphomas of particular diagnostic interest because the histological patterns were at variance with their immunophenotype. One of these lymphomas, involving the gallbladder and duodenum, showed a marginal zone lymphoma-like (MALT type) pattern of cellular infiltration with CD5 negativity but (unexpectedly) Cyclin D1 positivity. Fluorescence in situ hybridization analysis of this case was performed because of the aberrant expression of Cyclin D1, and was clearly positive for the Cyclin D1 gene translocation. The second case, occurring in a lymph node, showed the typical growth pattern of a follicular lymphoma but it had an atypical immunophenotype, namely, expression of Cyclin D1, CD10, and Bcl2 and focally Bcl6, accompanied by a lack of CD5 and CD23. The Cyclin D1 gene translocation was detected by fluorescence in situ hybridisation (FISH), whereas c-myc and Bcl2 genes translocation were absent. Numerical chromosomal changes, which were visualized for chromosomes 8, 11, and 18 could be correlated to the aberrant immunoprofile. In this context, we discuss the diagnostic value of Cyclin D1, CD5, CD23, CD10, Bcl6 markers revealed by immunohistochemistry, as well as the significance of detection by FISH of chromosomal translocations such as t(11;14) and t(14;18). The question still remains as to whether such cases should be designated as specific lymphoma entities or reported as unclassifiable and the chromosome aberration reported.
cyclin D1, differential diagnosis, FISH, immunohistochemistry, overlapping immunophenotype, small B-cell lymphomas
320-327
Kostopoulos, Ioannis
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Cocco, Mario
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Ginanneschi, Chiara
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D'Amuri, Alessandro
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Lazzi, Stefano
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Fabbri, Alberto
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Forconi, Francesco
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De Santi, Maria Margherita
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Leoncini, Lorenzo
ba95251c-885b-4881-b8fd-119bca42e9c2
September 2006
Kostopoulos, Ioannis
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Cocco, Mario
68f7e717-be93-48cc-ae09-a02750b332c5
Ginanneschi, Chiara
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D'Amuri, Alessandro
627157a4-685a-487d-9e4a-843131fce4f6
Lazzi, Stefano
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Fabbri, Alberto
01268e1d-6b5a-44e0-9702-a25caa971f59
Forconi, Francesco
ce9ed873-58cf-4876-bf3a-9ba1d163edc8
De Santi, Maria Margherita
fa596ddc-d04a-43f4-8cf9-552e76db7202
Leoncini, Lorenzo
ba95251c-885b-4881-b8fd-119bca42e9c2
Kostopoulos, Ioannis, Cocco, Mario, Ginanneschi, Chiara, D'Amuri, Alessandro, Lazzi, Stefano, Fabbri, Alberto, Forconi, Francesco, De Santi, Maria Margherita and Leoncini, Lorenzo
(2006)
Overlapping morphologic and immunophenotypic profiles in small B-cell lymphoma. A report of two cases.
Virchows Archiv, 449 (3), .
(doi:10.1007/s00428-006-0242-1).
(PMID:16847683)
Abstract
We present two cases of small B-cell lymphomas of particular diagnostic interest because the histological patterns were at variance with their immunophenotype. One of these lymphomas, involving the gallbladder and duodenum, showed a marginal zone lymphoma-like (MALT type) pattern of cellular infiltration with CD5 negativity but (unexpectedly) Cyclin D1 positivity. Fluorescence in situ hybridization analysis of this case was performed because of the aberrant expression of Cyclin D1, and was clearly positive for the Cyclin D1 gene translocation. The second case, occurring in a lymph node, showed the typical growth pattern of a follicular lymphoma but it had an atypical immunophenotype, namely, expression of Cyclin D1, CD10, and Bcl2 and focally Bcl6, accompanied by a lack of CD5 and CD23. The Cyclin D1 gene translocation was detected by fluorescence in situ hybridisation (FISH), whereas c-myc and Bcl2 genes translocation were absent. Numerical chromosomal changes, which were visualized for chromosomes 8, 11, and 18 could be correlated to the aberrant immunoprofile. In this context, we discuss the diagnostic value of Cyclin D1, CD5, CD23, CD10, Bcl6 markers revealed by immunohistochemistry, as well as the significance of detection by FISH of chromosomal translocations such as t(11;14) and t(14;18). The question still remains as to whether such cases should be designated as specific lymphoma entities or reported as unclassifiable and the chromosome aberration reported.
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Published date: September 2006
Keywords:
cyclin D1, differential diagnosis, FISH, immunohistochemistry, overlapping immunophenotype, small B-cell lymphomas
Organisations:
Cancer Sciences
Identifiers
Local EPrints ID: 358168
URI: http://eprints.soton.ac.uk/id/eprint/358168
ISSN: 0945-6317
PURE UUID: b704e742-4896-456a-a248-bca5fe36bdd3
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Date deposited: 22 Oct 2013 13:07
Last modified: 15 Mar 2024 03:41
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Author:
Ioannis Kostopoulos
Author:
Mario Cocco
Author:
Chiara Ginanneschi
Author:
Alessandro D'Amuri
Author:
Stefano Lazzi
Author:
Alberto Fabbri
Author:
Maria Margherita De Santi
Author:
Lorenzo Leoncini
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