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Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae

Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae
Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae
Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Polymerase Chain Reaction (PCR) utilizing published primers. The data was then compared with conventional serology using latex agglutination (LA) and the Quellung reaction. Based on the PCR-approach, 8 different serogroups/serotypes were detected with one isolate classified as non-typeable (cpsA-negative). In reference to the serology-based data, the results were in agreement except for one isolate. For the latter isolate, the LA and Quellung tests failed to show a reaction but the PCR-approach and sequencing identified the isolate as serogroup 15B/C. Based on this experimental setting, we found that the PCR-approach for pneumococcal serotypes determination is reliable to serve as the alternative for determining the pneumococcal serotyping.
0127-5720
338-344
Shakrin, N.N.S.M.
177ad22b-0d7d-4c48-aaed-ca145ca8cf49
Balasubramaniam, S.D.
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Yusof, H.A.
2f69bdb3-9eca-41a1-a962-34adb8bec664
Mastuki, M.F.
ace0d992-8525-4698-b61b-c45b45164392
Masri, S.N.
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Taib, N.M.
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Nordin, S.A.
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Jamal, F.
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Clarke, S.C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Desa, M.N.M.
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Shakrin, N.N.S.M.
177ad22b-0d7d-4c48-aaed-ca145ca8cf49
Balasubramaniam, S.D.
52b4d7f5-98d5-451c-ac7b-560396b0a48e
Yusof, H.A.
2f69bdb3-9eca-41a1-a962-34adb8bec664
Mastuki, M.F.
ace0d992-8525-4698-b61b-c45b45164392
Masri, S.N.
00dcb647-9aac-453e-9e9e-b722b3b00868
Taib, N.M.
66904393-9922-4fdf-842d-52f0b69d3e11
Nordin, S.A.
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Jamal, F.
11aded0c-7f6f-476a-997b-2e1dfcb3f9dc
Clarke, S.C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Desa, M.N.M.
b3fb3027-10b2-4f13-94d1-70753c0b5d64

Shakrin, N.N.S.M., Balasubramaniam, S.D., Yusof, H.A., Mastuki, M.F., Masri, S.N., Taib, N.M., Nordin, S.A., Jamal, F., Clarke, S.C. and Desa, M.N.M. (2013) Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae. Tropical Biomedicine, 30 (2), 338-344. (PMID:23959499)

Record type: Article

Abstract

Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Polymerase Chain Reaction (PCR) utilizing published primers. The data was then compared with conventional serology using latex agglutination (LA) and the Quellung reaction. Based on the PCR-approach, 8 different serogroups/serotypes were detected with one isolate classified as non-typeable (cpsA-negative). In reference to the serology-based data, the results were in agreement except for one isolate. For the latter isolate, the LA and Quellung tests failed to show a reaction but the PCR-approach and sequencing identified the isolate as serogroup 15B/C. Based on this experimental setting, we found that the PCR-approach for pneumococcal serotypes determination is reliable to serve as the alternative for determining the pneumococcal serotyping.

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More information

e-pub ahead of print date: June 2013
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 360021
URI: http://eprints.soton.ac.uk/id/eprint/360021
ISSN: 0127-5720
PURE UUID: 695187e4-566b-4e3e-af6f-d06d7c1b17ec
ORCID for S.C. Clarke: ORCID iD orcid.org/0000-0002-7009-1548

Catalogue record

Date deposited: 21 Nov 2013 12:56
Last modified: 09 Jan 2022 03:22

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Contributors

Author: N.N.S.M. Shakrin
Author: S.D. Balasubramaniam
Author: H.A. Yusof
Author: M.F. Mastuki
Author: S.N. Masri
Author: N.M. Taib
Author: S.A. Nordin
Author: F. Jamal
Author: S.C. Clarke ORCID iD
Author: M.N.M. Desa

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