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Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes

Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes
Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes
The first meiotic division of mammalian oocytes physiologically occurs in the ovary in the hours preceding ovulation. Fortunately, oocytes removed from their follicular environment will readily undergo this process in culture. Their large size, optical transparency, and efficiency in translating exogenous cRNA make mouse oocytes very amenable to study this process in detail using fluorescence imaging-based techniques. Here we describe the process of microinjecting cRNA to proteins of interest that have been coupled to a fluorescent protein using cyclin B1 as an example
978-1-62703-190-5
91-106
Humana Press
Holt, J.E.
a5879a86-a0c3-4c6e-87cc-54a65d97f0d8
Lane, S.I.R.
8e80111f-5012-4950-a228-dfb8fb9df52d
Jones, K.T.
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4
Holt, Jane E.
Lane, Simon I.R.
Jones, Keith T.
Holt, J.E.
a5879a86-a0c3-4c6e-87cc-54a65d97f0d8
Lane, S.I.R.
8e80111f-5012-4950-a228-dfb8fb9df52d
Jones, K.T.
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4
Holt, Jane E.
Lane, Simon I.R.
Jones, Keith T.

Holt, J.E., Lane, S.I.R. and Jones, K.T. (2012) Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes. In, Holt, Jane E., Lane, Simon I.R. and Jones, Keith T. (eds.) Mammalian Oocyte Regulation. (Methods in Molecular Biology, 957) New York, US. Humana Press, pp. 91-106. (doi:10.1007/978-1-62703-191-2_6).

Record type: Book Section

Abstract

The first meiotic division of mammalian oocytes physiologically occurs in the ovary in the hours preceding ovulation. Fortunately, oocytes removed from their follicular environment will readily undergo this process in culture. Their large size, optical transparency, and efficiency in translating exogenous cRNA make mouse oocytes very amenable to study this process in detail using fluorescence imaging-based techniques. Here we describe the process of microinjecting cRNA to proteins of interest that have been coupled to a fluorescent protein using cyclin B1 as an example

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Published date: 2012
Organisations: Centre for Biological Sciences

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Local EPrints ID: 361973
URI: http://eprints.soton.ac.uk/id/eprint/361973
ISBN: 978-1-62703-190-5
PURE UUID: c51e1e7f-1559-4da1-92b5-2fbbd6ac7e77
ORCID for S.I.R. Lane: ORCID iD orcid.org/0000-0002-8155-0981
ORCID for K.T. Jones: ORCID iD orcid.org/0000-0002-0294-0851

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Date deposited: 07 Feb 2014 16:31
Last modified: 15 Mar 2024 03:47

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Contributors

Author: J.E. Holt
Author: S.I.R. Lane ORCID iD
Author: K.T. Jones ORCID iD
Editor: Jane E. Holt
Editor: Simon I.R. Lane
Editor: Keith T. Jones

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